Vitamin ProfilingVitamin Profiling

Vitamin Profiling

Choose from a range of CE-IVD validated and complete assays for vitamin profiling. Calibrators and controls are also available separately. 

  • HPLC/UHPLC and LC-MS/MS assays available
  • Reliable methods with fast sample preparation
  • Calibrators and controls available separately 

Assays Available for Vitamin Profiling

Assays for the Combined Analysis of Vitamin A and E in Serum/Plasma

Order no. Method Number of tests /
sample preparations *
Run time (Column temperature)
Characteristics
34400 HPLC 200 • 2.9 min (35 °C)
• 3.7 min (ambient temperature)
Assay with Reaction Vials
34400-BK *
34400-DWP Assay with 96 Deep Well Plates
34400-DWP-BK *

* Basic Kit: designed for 200 sample preparations – not including mobile phase or calibrator

These assays are validated according to the new IVDR (=> Declaration of Conformity)


Order No. Method Number of tests Run time Characteristics
34700/F HPLC 5 x 96 9 min HPLC method, automated, with 96 Well Filter Plates
34900/F UHPLC 10 x 96 3.5 min UHPLC method, automated, with 96 Well Filter Plates

These assays are validated according to IVDD (=> Declaration of Conformity)


Download Brochure

Do you need all the facts at a glance? Then feel free to download our brochure vitamins A and E in serum/plasma on the brochure download page.

Assays for the Combined Analysis of Vitamin B1 and B6 in Whole Blood and Plasma/Serum

Order No. Method Specimen Number of tests Run time Characteristics
52052 HPLC Whole Blood,
B6 also Plasma
100 7 min (plasma)
9 min (whole blood)
Classic method
52052/Premix Simplified sample preparation with pre-mixed neutralisation tubes
52752/F Whole Blood 960 9 min HPLC method, with 96 Well Filter Plates
52952/UHPLC UHPLC Whole Blood,
B6 also Plasma
1000 3 min UHPLC method
52952/UHPLC/F
Whole Blood 960 UHPLC method, with 96 Well Filter Plates
87000 LC-MS/MS Whole Blood 480 2.5 min LC-MS/MS method, with Reaction Vials
87000/DWP LC-MS/MS method, with 96 Deep Well Plates

Assay for the Single Analysis of Vitamin B1 in Whole Blood

Order No. Method Specimen Number of tests Run time
35000 HPLC Whole Blood 100 6 min

Assays for the Single Analysis of Vitamin B6 in Whole Blood and Plasma/Serum

Order No. Method Specimen Number of tests Run time
31000/S HPLC Plasma/Serum 100 8 min
31000/WB Whole Blood

 

IVDR / IVDD
Our vitamin B1/B6 HPLC and UHPLC assays are already validated according to the new IVDR.
Our vitamin B1/B6 LC-MS/MS assay (87000) is validated according to the IVDD.

 

HPLC/UHPLC Solutions
Various methods for any requirements:
• Standard method or with Pre-mixed Tubes, for simplified manual sample preparation
• UHPLC methods for short analysis time and high sample throughput

 

LC-MS/MS Solution
Our mass spectrometry assay allows the determination of the active forms of vitamin B1 (TPP) and vitamin B6 (PLP) in whole blood by LC-MS/MS. The sample preparation can be performed very quickly and consists of a simple protein precipitation eliminating the need for a derivatisation – ideal for laboratories with higher throughputs.


Download Brochure

A good overview of all methods can be found in our brochures vitamins B1 and B6 with LC-MS/MS and vitamins B1 and B6 with HPLC and UHPLC, which you can download from the brochure download page.

Assay for the Analysis of Vitamin B2 in Whole Blood

Order No. Method Number of tests Analysis time Characteristics
37000 HPLC 100  < 9 min Simultaneous determination of riboflavin, FMN and FAD

Riboflavin (Vitamin B2) is the precursor of two coenzymes known as flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). Both are essential for tissue respiration and the generation of energy from the metabolism of carbohydrates, amino acids and fats. Riboflavin is vital for normal reproduction, growth, repair and development of body tissues including the skin, hair, nails, connective tissue and immune system. Riboflavin is mainly converted into FMN and FAD in the small intestine, liver, heart and kidney.

Assay for the Analysis of Vitamin C in Plasma/Serum

Order No. Method Number of tests Analysis time Characteristics
65065 HPLC 100 5 min Standard method

Vitamin C (ascorbic acid) is a water-soluble vitamin that is an essential cofactor for the human body, for instance in the catabolism of tyrosine and the synthesis of catecholamines. Regular consumption of fruit and vegetables is usually sufficient to cover the body’s vitamin C needs.

Assays for the Analysis of Vitamin D3/D2 in Serum/Plasma

Order No. Method Number of tests Run time Parameters
Characteristics
38038 HPLC 100 10 min 25-OH-Vitamin D2
25-OH-Vitamin D3
Standard HPLC method
38900/1000 HPLC 1000 10 min With online sample preparation
62000
62000/1000
LC-MS/MS 200
1000
5 – 6 min Standard LC-MS/MS method
62000/1000/F LC-MS/MS 1000 For sample preparation with 96 Well Filter Plates
62062
62062/1000
LC-MS/MS 200
1000
3.25 – 3.9 min 25-OH-Vitamin D2
25-OH-Vitamin D3
3-epi-25-OH-Vitamin D3
Shorter run time
62062/1000/F LC-MS/MS 1000 Shorter run time
For sample preparation with 96 Well Filter Plates

Vitamin D is a recognised clinical chemical determinant associated with osteoporosis and osteopenia (reduction in bone density). It also plays a role in the body's immune response, cardiovascular diseases and some types of cancer. Therefore, vitamin D is highly important from a number of diagnostic perspectives.

Assay for the Analysis of β-Carotene in Serum/Plasma

Order No. Method Number of tests Analysis time Characteristics
32000 HPLC 100  < 10 min Fast and easy sample prep

β-Carotene (Provitamin A) is a precursor form of vitamin A, and is an essential nutrient for the human diet. β-carotene is a herbal dye used as a colouring in the food industry and in vitamin products. In humans, all-trans-β-carotene is functionally and quantitatively the most important of about 20 carotenoids present in the blood plasma. This assay assures reliable separation of α-carotene, cis-β-carotene and all-trans-β-carotene as well as the carotenoids cryptoxanthin and lycopene.

Assay for the Analysis of Methylmalonic Acid in Serum/Plasma and Urine

Order No. Method Number of tests Analysis time Characteristics
64000 LC-MS/MS 400 3.5 min Clean-up tubes for fast processing of serum/plasma samples or
direct injection of urine samples after dilution
64000 Urine Upgrade Set LC-MS/MS 200 3.5 min Upgrade set for methylmalonic acid in urine

Methylmalonic acid (MMA) is an intermediate of amino acid metabolism which results from the conversion of propionyl-coenzyme A to succinyl-coenzyme A in a vitamin B12-dependent reaction, and is regarded as an early, sensitive biomarker of vitamin B12-deficiency. Vitamin B12-insufficiency leads to high concentrations of MMA in plasma, serum and urine. Our MassChrom® Methylmalonic Acid (MMA) kits allow the specific determination of methylmalonic acid in all three matrices.