34900-F UHPLC kit vitamin A vitamin E serum plasma

Automated Vitamins A and E in Serum/Plasma - UHPLC

Order no.: 34900/F
10 x 96 Tests
  • Vitamin A
  • Vitamin E

Automated sample preparation with 96 Well Filter Plates
96 samples in less than 30 minutes
Works on a range of liquid handling devices

Description

Clinical relevance

Vitamins A and E are fat-soluble vitamins, which perform important functions in the body.

Vitamin A plays an important role in vision, bone metabolism, and the synthesis of steroid hormones. Deficiency of vitamin A can lead to night blindness, skin dehydration, and hair loss.

Vitamin E protects, among others, unsaturated fatty acids in the cell membranes and the LDL cholesterol from attacks by reactive radicals, which can occur as a result of increased oxidative stress in the body. Deficiency of vitamin E can lead to permanent joint pain, making the determination of vitamin E levels essential in this case.

However, overdoses of these vitamins may cause hypervitaminosis with intoxication symptoms. In addition, chemotherapies should not be "accompanied" by extra vitamin supplements – especially high doses of vitamins A and E may limit the success of treatment.

 

Product advantages

  • Automated sample preparation:
    96 samples in less than 30 minutes

 

This UHPLC kit allows for the automated analysis of vitamins A and E in plasma/serum. The automated preparation of 96 samples can be completed in less than 30 minutes – ideal for resource-saving processing of larger numbers of samples. The UHPLC determination is performed in less than 4 minutes.

Chromsystems also offers another automated method using HPLC.

 

Kit content for 10 x 96 analyses:

Mobile Phase, 2 x 500 ml
Precipitation Reagent I, 4 x 2.5 ml
Internal Standard, 2 x 100 ml
Vitamins A and E Serum Calibration Standard (lyoph.), 5 x 1 ml
96 Well Filter Plates, 2 x 5 pcs.
96 Well Collection Plates, 2 x 5 pcs.
Pierceable Heat Seals for 96 Well Plates, 2 x 6 pcs.
UHPLC Column, 1 pc.
UHPLC Precolumn cartridge, 3 pcs.

Chromatogram

Technical Data

Method of Analysis

UHPLC

Number of Tests

10 x 96

Parameter

Vitamin A, Vitamin E

Analysis Time

3.5 min

Limit of quantification

Vitamin A: 0.02 mg/l
Vitamin E: 0.4 mg/l

Linearity

Vitamin A: 0.02-4.5 mg/l Vitamin E: 0.4-38 mg/l

Recovery

Vitamin A > 94 %; Vitamin E > 90 %

Intraassay

Vitamin A: CV < 5 %
Vitamin E: CV < 5 %

Interassay

Vitamin A: CV < 5 %
Vitamin E: CV < 5 %

Column temperature

approx. 35°C

Specimen

Serum/Plasma

Sample Preparation

  • Place 200 μl Internal Standard to the 96 well filter plate.
  • Add 50 μl sample/calibrator/control.
  • Add 10 μl Precipitation Reagent I.
  • Agitate the 96 well filter plate for 1 min.
  • Separate precipitate by centrifugation or suction (2 min at 1500 x g or 200 mbar, resp.).
  • Collect eluate in a collection plate.
  • Seal the collection plate with a heat seal and transfer to autosampler.

Sample Stability

Samples are stable up to 48 hours stored light protected in a tightly closable glass container at +2 to +8°C. For longer storage periods (up to 3 weeks) keep samples frozen below -18 °C.

Injection Volume

5 µl

Wavelength

325 nm, after 1.3 min switch to 295 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Calibrators

Calibrators available separately

Controls

Controls available separately

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