1003-B HPLC urine calibration standard VMA HVA 5-HIAA

Calibration Standard

Order no.: 1003/B
  • 5-Hydroxyindoleacetic acid (5-HIAA)
  • Homovanillic acid (HVA)
  • Vanillylmandelic acid (VMA)
VMA, HVA, 5-HIAA in Urine - HPLC


Calibration Standard for the analysis of VMA, HVA, 5-HIAA in urine, primary standard solution, lyophilised

This aqueous calibration standard (order no. 1003/B) from Chromsystems is injected directly into the HPLC system without sample preparation. While following the instructions the calibrator should be measured in the same manner as a patient specimen.

This aqueous calibrator is manufactured without using any human matrices.

An alternative is the lyophilised calibrator (order no. 1009), which is based on human urine and should be used according to the instruction manual.

Technical Data

Method of Analysis



5 x 1 ml (lyoph.)


5-Hydroxyindoleacetic acid (5-HIAA), Homovanillic acid (HVA), Vanillylmandelic acid (VMA)


Please note: All concentrations shown here are exemplatory. They do not reflect real data for the described products. Actual concentrations depend on the production lot of each product and can exclusively be found in the product information leaflet. Please ask for these product information leaflets or download them from the service site of the Chromsystems website.

Substance Value
Vanillylmandelic acid (VMA) 10.0
5-Hydroxyindole-3-acetic acid (5-HIAA) 9.64
Homovanillic acid (HVA) 9.92


Carefully remove the rubber stopper from the vial (Caution when removing the metal seal: Risk of injuries due to sharp cutting edges!). Add exactly 5.0 ml of distilled water into the vial. Replace the stopper and allow to sit for 10 to 15 minutes at room temperature. Swirl the vial to dissolve the contents until homogeneity

Storage conditions

Stored at +2 to +8 °C unopened calibration standards are stable until the expiry date which is stated on the vial labels.
The reconstituted calibration standard should be stored tightly capped at +2 to +8 °C.

Generally the calibration standard can be stored for two days at +2 to +8 °C. If the calibration standard cannot be analysed within this period, it should be aliquoted and stored frozen below –18 °C (up to 3 months).