VMA, HVA, 5-HIAA in Urine - HPLC

VMA, HVA, 5-HIAA in Urine - HPLC

Order no.: 1000/B
100 Tests
  • 5-Hydroxyindoleacetic acid (5-HIAA)
  • Homovanillic acid (HVA)
  • Vanillylmandelic acid (VMA)

No pH-adjustment necessary
Suitable for automated sample preparation
2 Internal standards available


Clinical Relevance

Neuroblastoma is a neoplastic disease that is among the most common malignant cancers occurring during childhood. It is a neuroendocrine tumour resulting in higher excretion of the catecholamine metabolites vanillylmandelic acid (VMA) and homovanillic acid (HVA) in the urine. Therefore verification of these parameters is critical in the diagnosis of neuroblastoma.

In contrast, increased concentrations of hydroxyindoleacetic acid (5-HIAA) detected in the urine are an indication of a carcinoid tumour. The malignant reproduction of enterochromaffin cells in the gastrointestinal tract leads to increased production of the tissue hormone serotonin, whose primary metabolite is 5-HIAA. 


Product  advantages

  • Simple and reliable sample preparation
  • Two internal standards if desired
  • Automated sample preparation for Gilson® ASPECTM is available


This kit enables routine HPLC determination of VMA, HVA and 5-HIAA in urine. Simple and safe operation guarantees reliable and reproducible results. An additional, optional internal standard (HICA) is also available for especially high precision in the determination of 5-HIAA.


Kit content

Mobile Phase, 1000 ml
VMA, HVA, 5-HIAA Calibration Standard (lyoph.), 5 x 1 ml
Internal Standard (iso-VMA), 100 ml
Wash Buffer I, 300 ml
Wash Buffer II, 300 ml
Elution Buffer, 200 ml
Finisher, 10 ml
Sample Clean Up Columns, 50 pcs.


Technical Data

Method of Analysis


Number of Tests



5-Hydroxyindoleacetic acid (5-HIAA), Homovanillic acid (HVA), Vanillylmandelic acid (VMA)

Analysis Time

< 18 min

Limit of quantification

VMA, HVA, 5-HIAA: 0.5 mg/l


VMA: up to 78 mg/l
HVA: up to 51 mg/l
5-HIAA: up to 78 mg/l


VMA: 70 %; HVA: 82 %; 5-HIAA: 66 %


CV < 4 %


CV < 5 %


24 h urine; spontaneous urine (refer the data to urine creatinine)

Pre-analytic Treatment

Determination of VMA/HVA

Collect urine in 10 ml HCl 25 %, pH < 4


Determination of 5-HIAA

Collect urine in 10 ml glacial acetic acid, pH 4 - 6

Sample Preparation

  • Extraction: Add 1 ml Internal Standard to 50 µl urine and mix. Apply entire sample to the sample clean up column, draw through by vacuum or centrifugation, discard effluent.
  • Washing: Apply 3 ml Wash Buffer I to the sample clean up column, discard effluent. Apply 2 times 3 ml Wash Buffer II, discard effluents.
  • Elution: Apply 2.0 ml Elution Buffer to the sample clean up column, collect eluate. Add 100 µl Finisher and mix briefly.
  • Inject 10–20 µl into the HPLC system.

Sample Stability

VMA, HVA: at +2 to +8 °C at least 5 days. 5-HIAA: at +2 to +8 °C in the dark max. 3 days. Aliquot and freeze below -18 °C for longer storage.

Additional Info

For the HPLC analysis of VMA, HVA and 5-HIAA in urine any isocratic HPLC system with electrochemical detector is suitable.

Injection Volume

10-20 µl

Flow rate

1 ml/min

Column temperature

ambient (~25 °C)


approx. +760 mV

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Mobile Phase

Order no.: 1011

Calibration Standard

Order no.: 1003/B

Wash Buffer I

Order no.: 1005

Wash Buffer II

Order no.: 1006

Elution Buffer

Order no.: 1077


Order no.: 1013


Calibrators available separately


Controls available separately