Vitamins B1/B6 Whole Blood Calibration Standard

Vitamins B1/B6 Whole Blood Calibration Standard

Order no.: 52003/RUO
  • Vitamin B1
  • Vitamin B6

Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC

Description

Vitamins B1/B6 Whole Blood Calibration Standard, lyophilised

This lyophilised calibrator from Chromsystems is based on human whole blood and should be used according to the instruction manual. It is designed to calibrate the measurement method for the quantitative determination of vitamin B1 and vitamin B6 (Pyridoxal-5-phosphate; Pyridoxal). While following the instructions the calibrator should be handled and measured in the same manner as research samples.

Technical Data

Method of Analysis

HPLC/UHPLC

Content

5 x 1 ml (lyoph.)

Parameter

Vitamin B1, Vitamin B6

Concentrations

Please note: Concentration values shown here are typical. For lot specific concentration values please refer to the product insert supplied with the materials. The product inserts can also be requested from us directly.

SubstanceUnitValue
Pyridoxal-5'-phosphate (PLP) µg/l
nmol/l
18.4
74.5
Thiamindiphosphate (TPP) µg/l
nmol/l
52.2
123

Manual

 

Reconstitution

Carefully remove the metal seal and rubber stopper from the vials. (Caution when removing the metal seal: risk of injuries due to sharp cutting edges!). Add exactly 1.0 ml of distilled water into each vial. Return the stopper and incubate for 10 to 15 minutes at room temperature. Swirl the vials to dissolve the contents until homogeneity.

Storage conditions

Stored below –18 °C and unopened, the lyophilised calibrator is stable until the expiry date specified on the vial labels. The reconstituted calibrator can be stored up to 24 hours light protected and tightly capped at +2 to +8 C. If the calibrator cannot be used within this period, it should be aliquoted and stored frozen below -18 °C (up to 3 months). For more details about storage conditions please check your instruction manual.

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