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Description
Vitamin B1/B2 Whole Blood Controls, lyophilised
0033/RUO Vitamin B1/B2 Whole Blood Control Bi-Level I + II (lyoph.), 2 x 5 x 2 ml
0034/RUO Vitamin B1/B2 Whole Blood Control Level I (lyoph.), 5 x 2 ml
0035/RUO Vitamin B1/B2 Whole Blood Control Level II (lyoph.), 5 x 2 ml
These lyophilised Chromsystems controls are based on human whole blood and should be used according to the instruction manual. They are designed to monitor the accuracy and precision of analytical procedures for the quantitative determination of Vitamin B1 and Vitamin B2. While following the instructions the controls should be handled and measured in the same manner as research samples.
Technical Data
Method of Analysis
HPLC
Concentrations
Please note: Concentration values shown here are typical. For lot specific concentration values please refer to the product insert supplied with the materials. The product inserts can also be requested from us directly.
| Substance | Unit | Suggested value Level I | Suggesed range Level I | Suggested value Level II | Suggested range Level II |
|---|---|---|---|---|---|
| Thiamindiphosphate (TPP) | µg/l nmol/l |
24.7 58.1 |
17.3-32.1 40.7-75.6 |
99.4 234 |
69.6-129 164-305 |
| Flavinadeninedinucleotide (FAD) | µg/l nmol/l |
103 131 |
82.5-124 105-158 |
366 466 |
293-439 372-559 |
| Flavinmononucleotide (FMN) | µg/l nmol/l |
3.8 8.32 |
2.66-4.94 5.83-10.8 |
7.02 15.4 |
4.92-9.13 10.8-20.0 |
| Riboflavin | µg/l nmol/l |
8.93 23.7 |
7.14-10.7 19.0-28.5 |
33.4 88.7 |
26.7-40.1 70.9-106 |
| Total riboflavin | µg/l nmol/l |
61.5 163 |
49.2-73.8 131-196 |
214 570 |
171-257 456-683 |
Reconstitution
Carefully remove the metal seal and rubber stopper from the vials. (Caution when removing the metal seal: risk of injuries due to sharp cutting edges!). Add exactly 2.0 ml of distilled water into each vial. Return the stopper and incubate for 10 to 15 minutes at room temperature. Swirl the vials to dissolve the contents until homogeneity.
Storage conditions
Stored below -18 °C and unopened, the lyophilised controls are stable until the expiry date specified on the vial labels. The reconstituted controls can be stored up to 24 hours light protected and tightly capped at +2 to +8 C. If the controls cannot be used within this period, they should be aliquoted and stored frozen below –18 °C (up to 3 months). For more details about storage conditions please check your instruction manual.
