Porphyrins in Urine - HPLC

Porphyrins in Urine - HPLC

Order no.: 44000
100 Tests
  • Coproporphyrin I
  • Coproporphyrin III
  • Heptacarboxyporphyrin
  • Hexacarboxyporphyrin
  • Pentacarboxyporphyrin
  • Uroporphyrin

Easy sample preparation
Specifically developed internal standard
Stable standards and controls


Clinical relevance

Porphyrins are precursors of haem and usually only occur in urine in negligible amounts. However, disorders in haem biosynthesis lead to the syndrome of porphyria with increased formation and excretion of porphyrins. Porphyrias can be hereditary-related or acquired through exogenous influences such as alcohol consumption or lead poisoning. Different enzymes of the haem biosynthesis can be deficient, thus, several different porphyrias can be distinguished. Since these share some common symptoms, chromatographic measurement of the porphyrins is the only way to enable an exact diagnosis.


Product advantages

  • Easy sample preparation
  • Custom-made internal standard
  • Stable standards and controls


This kit is designed for the fast and reliable determination of all porphyrins in urine that are relevant to differential diagnosis. Sample preparation requires only the addition of an internal standard and a precipitation reagent, followed by the centrifugation of the urine. The internal standard elutes in the middle of the chromatogram, making it easier to interpret the results of the measurement.


Kit content

Mobile Phase A, 1000 ml
Mobile Phase B, 1000 ml
Porphyrins Urine Calibration Standard (lyoph.), 5 x 2 ml
Internal Standard, 5 ml
Stabilisation Reagent, 5 ml
Priming Solution, 5 ml
Reaction vials, amber coloured (light protection), 100 pcs.


Technical Data

Method of Analysis


Number of Tests



Coproporphyrin I, Coproporphyrin III, Heptacarboxyporphyrin, Hexacarboxyporphyrin, Pentacarboxyporphyrin, Uroporphyrin

Analysis Time

15 min + approx. 5 min reequilibration

Limit of quantification

3 µg/l


up to 5000 µg/l


100–106 %


CV ≤ 3.9 %


CV ≤ 6 %



Pre-analytic Treatment

Keep patient specimens cool and light protected during transport. At a pH value of 6 to 7 storage life is up to 2 days at +2 to +8 °C. For longer storage (up to 1 month) keep samples below -18 °C. Stabilize acidic urines by sodium hydrogen carbonate (attention: foaming). Avoid repeated freeze/thaw cycles!

Sample Preparation

  • Pipette 500 µl urine into a light-protected reaction vial.
  • Add 50 µl Stabilisation Reagent and 50 µl Priming Solution, mix briefly (vortex); incubate 10 min at ambient temperature.
  • Add 50 µl Internal Standard, mix briefly (vortex).
  • Centrifuge 10 min at 9000 x g.
  • Inject 25 µl supernatant into the HPLC system.

Sample Stability

The prepared samples are stable at ambient temperature at least for 24 h, at +2 to +8 °C or below -18 °C up to 6 days.

Additional Info

Any binary HPLC gradient system with fluorescence detector is suitable.

Injection Volume

25 µl

Flow rate

1.2 ml/min



Column temperature

ambient (+18 to +30 °C)


EX 405 nm
EM 620 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Mobile Phase A

Order no.: 44001

Mobile Phase B

Order no.: 44002

Internal Standard

Order no.: 44004

Stabilisation Reagent

Order no.: 44005

Priming Solution

Order no.: 44006

Reaction vials

Order no.: 33005


Calibrators available separately


Controls available separately