Parameter Set Mycophenolic Acid - LC-MS/MS

Parameter Set Mycophenolic Acid - LC-MS/MS

  • Mycophenolic acid
  • Mycophenolic acid glucuronide

Order no.: 92916/RUO, 92111/RUO, 92110/RUO

For the analysis of MPA and MPAG
6PLUS1® Multilevel Calibrator Set available
Part of the modular system of the Series A

Product Name Qty
Parameter Set - Mycophenolic Acid - LC-MS/MS
Order no.: 92916/RUO
BASIC Kit A for 200 tests - LC-MS/MS
Order no.: 92111/200/RUO
BASIC Kit A for 1000 tests - LC-MS/MS
Order no.: 92111/1000/RUO
MassTox® MasterColumn® A
Order no.: 92110/RUO


The modular system MassTox® Basic Kit A in combination with the MassTox® Parameter Set for Mycophenolic Acid allows for the fast quantitative determination of mycophenolic acid and its glucuronide in serum/plasma by LC-MS/MS. The use of stable isotopically marked, co-eluating internal standards and the 6PLUS1® multilevel calibrators ensures high performance of the analysis method.


Technical Data

Method of Analysis



Mycophenolic acid, Mycophenolic acid glucuronide

Analysis Time

1.8 min


Starting point       60 % Mobile Phase 2
0.00→0.50 min    100 % Mobile Phase 2
0.51–1.00 min    100 % Mobile Phase 2
1.01→1.10 min     60 % Mobile Phase 2
1.11–1.81 min      60 % Mobile Phase 2



Sample Preparation

  • Prepare the Internal Standard Mix
  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 μl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 μl Extraction Buffer
  • Mix and incubate 2 min at ambient temperature (do not centrifuge)
  • Add 250 μl of mixture A and mix 30 s minimum (vortex)
  • Centrifuge 5 min at 15000 x g.
  • Dilute the supernatant with Dilution Buffer prior to injection, depending on the instrument sensitivity

Sample Stability

Research samples are stable up to 4 weeks at +2 to +8 °C. For longer storage periods keep samples frozen below -18 °C.

Injection Volume

0.2 – 50 µl


ESI positive



Additional Info

We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components