Parameter Set - Mycophenolic Acid  - LC-MS/MS

Parameter Set - Mycophenolic Acid - LC-MS/MS

Order no.: 92916/RUO
  • Mycophenolic acid
  • Mycophenolic acid glucuronide

For the analysis of MPA and MPAG
6PLUS1® Multilevel Calibrator Set available
Part of the modular system of the Series A

Description

The modular system MassTox® Basic Kit A in combination with the MassTox® Parameter Set for Mycophenolic Acid allows for the fast quantitative determination of mycophenolic acid and its glucuronide in serum/plasma by LC-MS/MS. The use of stable isotopically marked, co-eluating internal standards and the 6PLUS1® multilevel calibrators ensures high performance of the analysis method.

For the analysis you require the MassTox® Basic Kit A, the specifc MassTox® Parameter Set and the analytical column MassTox® MasterColumn® A.

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Parameter

Mycophenolic acid, Mycophenolic acid glucuronide

Analysis Time

1.8 min

Specimen

Serum/Plasma

Sample Preparation

  • Reconstitute the Internal Standard Mix.
  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 μl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 μl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 μl of mixture A and mix 30 s minimum (vortex) and centrifuge 5 min.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity

Sample Stability

Stability of research samples depends on the specific analyte. Further information can be optained from the instruction manual.

Injection Volume

0.2 – 50 µl

Gradient

Starting point       60 % Mobile Phase 2
0.00→0.50 min    100 % Mobile Phase 2
0.51–1.00 min    100 % Mobile Phase 2
1.01→1.10 min     60 % Mobile Phase 2
1.11–1.81 min      60 % Mobile Phase 2

Ionisation

ESI positive

MS/MS-Mode

MRM

Additional Info

We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Calibrators

Controls