Parameter Set - Antiepileptic Drugs - LC-MS/MS

Parameter Set - Antiepileptic Drugs - LC-MS/MS

Order no.: 92921/RUO
  • 10,11-Dihydroxycarbamazepine
  • 10-OH-carbamazepine
  • Carbamazepine
  • Carbamazepine-10,11-epoxide
  • Ethosuximide
  • Felbamate
  • Gabapentin
  • Lacosamide
  • Lamotrigine
  • Levetiracetam (Keppra®)
  • N-Desmethylmesuximide
  • Oxcarbamazepine
  • Phenobarbital
  • Phenylethylmalonamide (PEMA)
  • Phenytoin
  • Pregabalin
  • Primidone
  • Rufinamide
  • Stiripentol
  • Sultiame
  • Theophylline
  • Tiagabine
  • Topiramate
  • Valproic acid
  • Vigabatrin
  • Zonisamide

Encompasses 26 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the modular system of the Series A

Description

The modular system MassTox® Basic Kit A in combination with the MassTox® Parameter Set for Antiepileptic Drugs allows for the fast quantitative determination of 26 different analytes in serum/plasma by LC-MS/MS. The use of stable isotopically marked and co-eluating internal standards ensures high performance of the analysis method.

For the analysis you require the MassTox® Basic Kit A, the specifc MassTox® Parameter Set and the analytical column MassTox® MasterColumn® A.



Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Parameter

10,11-Dihydroxycarbamazepine, 10-OH-carbamazepine, Carbamazepine, Carbamazepine-10,11-epoxide, Ethosuximide, Felbamate, Gabapentin, Lacosamide, Lamotrigine, Levetiracetam (Keppra®), N-Desmethylmesuximide, Oxcarbamazepine, Phenobarbital, Phenylethylmalonamide (PEMA), Phenytoin, Pregabalin, Primidone, Rufinamide, Stiripentol, Sultiame, Theophylline, Tiagabine, Topiramate, Valproic acid, Vigabatrin, Zonisamide

Analysis Time

2.5 – 3.5 min

Specimen

Serum/Plasma

Sample Preparation

  • Add 800 µl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 µl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 µl Extraction Buffer, mix briefly (vortex) and incubate 2 min
  • Add 250 µl of mixture A, mix 30 s (vortex) and centrifuge 5 min
  • Dilute supernatant with Dilution Buffer prior to injection, depending on instrument sensitivity.

Sample Stability

Stability of research samples depends on the specific analyte. Further information can be optained from the instruction manual.

Injection Volume

0.2 – 50 µl

Gradient

Starting point: 0% Mobile Phase 2

Group 1
0.00→2.00 min, 100 % Mobile Phase 2
2.00-2.60 min, 100 % Mobile Phase 2
2.61-3.50 min, 0 % Mobile Phase 2

Group 2 and 3
0.00→1.00 min, 100 % Mobile Phase 2
1.00-1.60 min, 100 % Mobile Phase 2
1.61-2.50 min, 0 % Mobile Phase 2

Group 4 and 5
0.00→1.00 min, 100 % Mobile Phase 2
1.00-1.80 min, 100 % Mobile Phase 2
1.81-2.70 min, 0 % Mobile Phase 2

Ionisation

ESI positive and negative

MS/MS-Mode

MRM

Additional Info

We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Calibrators

Calibrators available separately

Controls