Parameter Set Antiepileptic Drugs - LC-MS/MS

Parameter Set Antiepileptic Drugs - LC-MS/MS

   
  • 10,11-Dihydroxycarbamazepine
  • 10-OH-carbamazepine
  • Carbamazepine
  • Carbamazepine-10,11-epoxide
  • Ethosuximide
  • Felbamate
  • Gabapentin
  • Lacosamide
  • Lamotrigine
  • Levetiracetam (Keppra®)
  • N-Desmethylmesuximide
  • Oxcarbamazepine
  • Phenobarbital
  • Phenylethylmalonamide (PEMA)
  • Phenytoin
  • Pregabalin
  • Primidone
  • Rufinamide
  • Stiripentol
  • Sultiame
  • Theophylline
  • Tiagabine
  • Topiramate
  • Valproic acid
  • Vigabatrin
  • Zonisamide

Order no.: 92921/RUO, 92111/RUO, 92110/RUO

Encompasses 26 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the modular system of the Series A

Product Name Qty
Parameter Set - Antiepileptic Drugs - LC-MS/MS
Order no.: 92921/RUO
BASIC Kit A for 200 tests - LC-MS/MS
Order no.: 92111/200/RUO
BASIC Kit A for 1000 tests - LC-MS/MS
Order no.: 92111/1000/RUO
MassTox® MasterColumn® A
Order no.: 92110/RUO

Description

The modular system MassTox® Basic Kit A in combination with the MassTox® Parameter Set for Antiepileptic Drugs allows for the fast quantitative determination of 26 different analytes in serum/plasma by LC-MS/MS. The use of stable isotopically marked and co-eluating internal standards ensures high performance of the analysis method.

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Parameter

10,11-Dihydroxycarbamazepine, 10-OH-carbamazepine, Carbamazepine, Carbamazepine-10,11-epoxide, Ethosuximide, Felbamate, Gabapentin, Lacosamide, Lamotrigine, Levetiracetam (Keppra®), N-Desmethylmesuximide, Oxcarbamazepine, Phenobarbital, Phenylethylmalonamide (PEMA), Phenytoin, Pregabalin, Primidone, Rufinamide, Stiripentol, Sultiame, Theophylline, Tiagabine, Topiramate, Valproic acid, Vigabatrin, Zonisamide

Analysis Time

2.5 - 3.5 min

Gradient

Starting point: 0% Mobile Phase 2

Group 1
0.00→2.00 min, 100 % Mobile Phase 2
2.00-2.60 min, 100 % Mobile Phase 2
2.61-3.50 min, 0 % Mobile Phase 2

Group 2 and 3
0.00→1.00 min, 100 % Mobile Phase 2
1.00-1.60 min, 100 % Mobile Phase 2
1.61-2.50 min, 0 % Mobile Phase 2

Group 4 and 5
0.00→1.00 min, 100 % Mobile Phase 2
1.00-1.80 min, 100 % Mobile Phase 2
1.81-2.70 min, 0 % Mobile Phase 2

Specimen

Serum/Plasma

Sample Preparation

  • Add 800 µl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 µl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 µl Extraction Buffer, mix briefly (vortex) and incubate 2 min
  • Add 250 µl of mixture A, mix 30 s (vortex) and centrifuge 5 min
  • Dilute supernatant with Dilution Buffer (depending on instrument sensitivity) and inject into LC-MS/MS system

Sample Stability

Research samples are stable up to 1 week at +2 to +8 °C. For longer storage periods keep samples frozen below -18 °C.

Injection Volume

0.2–50 µl

Ionisation

ESI positive and negative

MS/MS-Mode

MRM

Additional Info

We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Calibrators

Calibrators available separately

Controls

Controls available separately