Parameter Set Anti-HIV Drugs - LC-MS/MS

Parameter Set Anti-HIV Drugs - LC-MS/MS

   
  • Amprenavir
  • Atazanavir
  • Darunavir
  • Delavirdine
  • Efavirenz
  • Elvitegravir
  • Etravirine
  • Indinavir
  • Lopinavir
  • Maraviroc
  • Nelfinavir
  • Nelfinavir-M8
  • Nevirapine
  • Raltegravir
  • Rilpivirine
  • Ritonavir
  • Saquinavir
  • Tipranavir

Order no.: 92924/RUO, 92111/RUO, 92110/RUO

Encompasses 18 analytes
6PLUS1® Multilevel Calibrator Set available
Part of the modular system of the Series A

Product Name Qty
Parameter Set - Anti-HIV Drugs
Order no.: 92924/RUO
BASIC Kit A for 200 tests - LC-MS/MS
Order no.: 92111/200/RUO
BASIC Kit A for 1000 tests - LC-MS/MS
Order no.: 92111/1000/RUO
MassTox® MasterColumn® A
Order no.: 92110/RUO

Description

The modular system MassTox® BASIC Kit A in combination with the MassTox® PARAMETER Set for Anti-HIV Drugs allows for the fast quantitative determination of amprenavir, atazanavir, darunavir, delavirdine, efavirenz, elvitegravir, etravirine, indinavir, lopinavir, maraviroc, nelfinavir, nelfinavir-M8, nevirapine, raltegravir, rilpivirine, ritonavir, saquinavir and tipranavir in serum/plasma by LC-MS/MS. The use of stable isotopically marked, co-eluating internal standards and the 6PLUS1® multilevel calibrator set ensures high performance of the analysis method.

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Parameter

Amprenavir, Atazanavir, Darunavir, Delavirdine, Efavirenz, Elvitegravir, Etravirine, Indinavir, Lopinavir, Maraviroc, Nelfinavir, Nelfinavir-M8, Nevirapine, Raltegravir, Rilpivirine, Ritonavir, Saquinavir, Tipranavir

Analysis Time

≤ 3.5 min

Gradient

Group 1
0.00–0.20 min       0 % Mobile Phase 2
0.21–2.00 min     70 % Mobile Phase 2
2.01–2.90 min   100 % Mobile Phase 2
2.91–3.50 min       0 % Mobile Phase 2
Group 2
0.00–0.20 min       0 % Mobile Phase 2
0.21–0.60 min     50 % Mobile Phase 2
0.61–2.40 min     90 % Mobile Phase 2
2.41–3.00 min       0 % Mobile Phase 2
Group 3
0.00–0.20 min       0 % Mobile Phase 2
0.21–2.30 min     90 % Mobile Phase 2
2.31–3.10 min       0 % Mobile Phase 2

Specimen

Serum/Plasma

Sample Preparation

  • Reconstitute the Internal Standard Mix.
  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A.
  • Pipette 50 μl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial.
  • Add 25 μl Extraction Buffer.
  • Mix and incubate 2 min at ambient temperature (do not centrifuge).
  • Add 250 μl of mixture A and mix 30 s minimum (vortex).
  • Centrifuge 5 min at 15000 x g.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity.

Sample Stability

Protected from light and tightly capped the research samples are stable 3 days at ambient temperature (ca. 25°C) and 5 days refrigerated (+2 to +8°C).

Injection Volume

15 - 25 μl

Ionisation

ESI (positiv)

MS/MS-Mode

MRM

Additional Info

We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Calibrators

Calibrators available separately

Controls

Controls available separately