One Step Vitamins A and E in Serum/Plasma - HPLC

One Step Vitamins A and E in Serum/Plasma - HPLC

Order no.: 34000/Premix
100 Tests
  • Vitamin A
  • Vitamin E

With ready-to-use pre-mixed tubes
Only one pipetting step
Fully automated workflow available


Clinical relevance

Vitamins A and E are fat-soluble vitamins, which perform important functions in the body.

Vitamin A plays an important role in vision, bone metabolism, and the synthesis of steroid hormones. Deficiency of vitamin A can lead to night blindness, skin dehydration, and hair loss.

Vitamin E protects, among others, unsaturated fatty acids in the cell membranes and the LDL cholesterol from attacks by reactive radicals, which can occur as a result of increased oxidative stress in the body. Deficiency of vitamin E can lead to permanent joint pain, making the determination of vitamin E levels essential in this case.

However, overdoses of these vitamins may cause hypervitaminosis with intoxication symptoms. In addition, chemotherapies should not be "accompanied" by extra vitamin supplements – especially high doses of vitamins A and E may limit the success of treatment.


Product advantages

  • Extremely simple sample preparation in a single step
  • Easy handling
  • Low analysis costs
  • Automated version available


This Chromsystems kit allows the simultaneous analysis of vitamins A and E in serum/plasma in a single isocratic HPLC run with UV detection. The special kit feature: Sample preparation is performed very fast and simply in one single pipetting step using premixed tubes. Additionally, a stable internal standard is used for precise quantification.

Chromsystems offers additional kits for the fast and efficient determination of vitamins A and E. You can choose between several options depending on the device capabilities (HPLC or UHPLC) and the sample quantities in your laboratory. Alternatively, the standard kit 34000 is available, as well as the 34900/UHPLC kit for higher sample throughput using UHPLC determination. Our kits are also available with automated workflow (34700/F and 34900/F).


Technical Data

Method of Analysis


Number of Tests



Vitamin A, Vitamin E

Analysis Time

approx. 9 min

Limit of quantification

Vitamin A: 0.02 mg/l
Vitamin E: 0.60 mg/l


Vitamin A: up to 4.5 mg/l
Vitamin E: up to 38 mg/l


Vitamin A > 99 %; Vitamin E > 96 %


Vitamin A: CV ≤ 4.9 %

Vitamin E: CV ≤ 4.1 %


Vitamin A: CV ≤ 5.2 %

Vitamin E: CV ≤ 3.8 %



Pre-analytic Treatment

Time of sample drawing

  • In the morning before breakfast
  • Prior to any medication

Collection and storage of specimens

  • Collect about 1 to 2 ml blood.
  • Centrifugate 5 min at 2000 x g.
  • Store serum/plasma below -18 °C and protect from light until analysed.

Sample Preparation

  • Pipette 100 µl serum/plasma into a Pre-mixed Reaction Tube.
  • Mix 30 s (vortex)
  • Centrifuge 10 min at at least 9000 x g.
  • Transfer supernatant into a light protected autosampler vial
  • inject 50 µl into the HPLC system.

Sample Stability

Protect prepared samples from light. At +2 to +8°C stable up to 48 h and at +20 to +25 °C up to 24 h. For longer storage (max. 3 weeks) keep the samples below -18 °C.

Additional Info

For the analysis of vitamins A and E any isocratic HPLC system with UV detector is suitable. As these vitamins are detected at different wavelengths, a detector with programmable wavelength switching is very useful for simultaneous analysis of vitamins A and E in a single HPLC run.

Injection Volume

50 µl

Flow rate

1.50 ml/min



Column temperature

ambient (~ 25 °C)


325 nm, after 3.5 min switch to 295 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately


Calibrators available separately


Controls available separately