Parameter Set Antimycotics - LC-MS/MS

Parameter Set Antimycotics - LC-MS/MS

  • 5-Flucytosine
  • Fluconazole
  • Hydroxyitraconazole
  • Itraconazole
  • Ketoconazole
  • Posaconazole
  • Voriconazole

Order no.: 92922/RUO, 92111/RUO, 92110/RUO

Encompasses 7 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the modular system of the Series A

Product Name Qty
Parameter Set - Antimycotic Drugs
Order no.: 92922/RUO
BASIC Kit A for 200 tests - LC-MS/MS
Order no.: 92111/200/RUO
BASIC Kit A for 1000 tests - LC-MS/MS
Order no.: 92111/1000/RUO
MassTox® MasterColumn® A
Order no.: 92110/RUO


The modular system MassTox® Basic Kit A in combination with the MassTox® Parameter Set for Antimycotic Drugs allows for the fast quantitative determination of fluconazole, 5-flucytosine, hydroxyitraconazole, itraconazole, ketoconazole, posaconazole and voriconazole in serum/plasma by LC-MS/MS. The use of stable isotopically marked and co-eluating internal standards ensures high performance of the analysis method.


Technical Data

Method of Analysis



5-Flucytosine, Fluconazole, Hydroxyitraconazole, Itraconazole, Ketoconazole, Posaconazole, Voriconazole

Analysis Time

3.2 min


0.00 - 0.50 min 30 % Mobile Phase 2
0.51 - 2.80 min 100 % Mobile Phase 2
2.81 - 3.20 min 30 % Mobile Phase 2



Sample Preparation

  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A.
  • Pipette 50 μl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial.
  • Add 25 μl Extraction Buffer.
  • Mix and incubate 2 min at ambient temperature (do not centrifuge).
  • Add 250 μl of mixture A and mix 30 s minimum (vortex).
  • Centrifuge 5 min at 15000 x g.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity

Sample Stability

Protected from light and tightly capped the research samples are stable up to 1 week at +2 to +8°C. For longer storage keep them frozen below -18°C.

Injection Volume

0.2 – 50 µl


ESI (positiv)



Additional Info

We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components


Calibrators available separately


Controls available separately