MassChrom® Steroids in Serum/Plasma with Sample Clean Up Columns - LC-MS/MS

MassChrom® Steroids in Serum/Plasma with Sample Clean Up Columns - LC-MS/MS

Order no.: 72072/C/RUO
96 Tests
  • 11-Deoxycorticosterone
  • 11-Deoxycortisol
  • 17-OH-progesterone
  • 21-Deoxycortisol
  • Aldosterone
  • Androstenedione
  • Corticosterone
  • Cortisol
  • Cortisone
  • Dehydroepiandrosterone (DHEA)
  • Dehydroepiandrosterone sulfate (DHEA-S)
  • Dihydrotestosterone (DHT)
  • Estradiol
  • Progesterone
  • Testosterone

Optimised and extended method
15 Steroids – 2 panels – 1 analytical column
With Sample Clean Up Columns

Description

This MassChrom® Steroid kit allows the quantitative determination of 15 steroids in serum/plasma by LC-MS/MS. Sample preparation is the same for both steroid panels using an optimised and efficient solid phase extraction with individual SPE columns (Sample Clean Up Columns). The use of stable isotope-labelled internal standards for every single analyte ensures reproducible and reliable quantitation of the steroids. The method does not require a high-end LC-MS/MS system to be carried out.

Alternatively, there is also a method available using 96 SPE well plates (72072/96/RUO, 72072/480/RUO).

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Number of Tests

96

Parameter

11-Deoxycorticosterone, 11-Deoxycortisol, 17-OH-progesterone, 21-Deoxycortisol, Aldosterone, Androstenedione, Corticosterone, Cortisol, Cortisone, Dehydroepiandrosterone (DHEA), Dehydroepiandrosterone sulfate (DHEA-S), Dihydrotestosterone (DHT), Estradiol, Progesterone, Testosterone

Analysis Time

Steroid Panel 1: 10.0 min; Steroid Panel 2: 11.7 min

Gradient

binary

Column temperature

Steroid Panel 1: 32 °C, Steroid Panel 2: 27 °C

Specimen

Serum/Plasma

Sample Preparation

  • Put Sample Clean Up Columns into tubes for sample preparation.
  • Pipette 0.8 ml Equilibration Reagent 1 into each Sample Clean Up Column, centrifuge and repeat with Equilibration Reagent 2.
  • Pipette 500 μl research sample/calibrator/MassCheck® control, 50 μl Internal Standard Mix and 450 μl Extraction Buffer into each Sample Clean Up Column, mix briefly (vortex) and centrifuge.
  • Add 2 x 0.7 ml Wash Buffer and centrifuge.
  • Put autosampler vials into tubes for sample preparation, then put Sample Clean Up Columns on top.
  • Add 1 ml Elution Buffer and centrifuge.
  • Evaporate eluates in the autosampler vials (nitrogen or compressed air) to dryness.
  • Add 100 μl Reconstitution Buffer.
  • Close autosampler vials, mix briefly (vortex) and inject 5–50 μl of the eluate.  

Sample Stability

Stability of research samples depends on the specific steroid. Further information can be obtained from the instruction manual.

Injection Volume

5-50 µl

Ionisation

ESI negative and positive (ESI switch)

MS/MS-Mode

MRM

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Mobile Phase A


Order no.: 72011/RUO

Mobile Phase B


Order no.: 72002/RUO

Elution Buffer


Order no.: 72033/RUO

Internal Standard Mix


Order no.: 72044/RUO

Extraction Buffer


Order no.: 72005/RUO

Reconstitution Buffer


Order no.: 72006/RUO

Equilibration Reagent 1


Order no.: 72077/RUO

Equilibration Reagent 2


Order no.: 72008/RUO

Rinsing Solution


Order no.: 72009/RUO

Wash Buffer


Order no.: 72012/RUO

Sample Clean Up Columns


Order no.: 72055/RUO

Calibrators

Controls