80700-C 80700-96 LCMS kit biogenic amines urine total metanephrine normetanephrine 3-methoxyryramine

MassChrom® Free Metanephrines in Plasma - LC-MS/MS

   
  • 3-Methoxytyramine
  • Metanephrine
  • Normetanephrine

Order no.: 81000/C, 81000
2x96 / 2x96 Tests

With Sample Clean Up Columns or 96 SPE Well Plates

Product Name Qty
MassChrom® Free Metanephrines in Plasma with Sample Clean Up Columns
Order no.: 81000/C
MassChrom® Free Metanephrines in Plasma with 96 SPE Well Plate
Order no.: 81000

Description

Clinical relevance

These kits allow the quantitative determination of metanephrine, normetanephrine and 3-methoxytyramine in plasma by LC-MS/MS.

One typical application for these kits is the analysis of patient samples with a suspicion of pheochromocytomas. These tumours and other neural crest tumours (e.g. paragangliomas and neuroblastomas) secrete metanephrine, normetanephrine and 3-methoxytyramine. The plasma free metanephrines are more stable and secreted more evenly than catecholamines (epinephrine, norepinephrine, and dopamine). Some studies show that the analysis of plasma free metanephrines has the highest sensitivity and specificity in pheochromocytoma diagnostics compared to plasma free catecholamines and metanephrines in urine.


 

Product advantages

  • 6PLUS1® Multilevel Calibrator Set and MassCheck® controls based on human plasma
  • Simple sample prep in just a few steps
  • Chromatographic separation of analytes – no co-elution
  • Isotopically labelled internal standards for all analytes
  • Different configurations available depending on your requirements:
    - with Sample Clean Up Columns (81000/C), for manual sample preparation
    - with 96 SPE Well Plates (81000), suitable for manual and automated sample preparation

 

Our kits encompass all required components and allow a simple and fast sample preparation: solid phase extraction (SPE) without equilibration/conditioning steps, analyte enrichment without evaporation step, only one elution step. The analytes are chromatographically separated to avoid co-elution and to minimise risks of interferences. The use of stable isotope-labelled internal standards for every single analyte ensures reproducible and reliable quantification. The 6PLUS1® Multilevel Plasma Calibrator Set and MassCheck® controls ensure high precision of results. They are obtained from human plasma to minimise matrix-induced differences.

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Parameter

3-Methoxytyramine, Metanephrine, Normetanephrine

Specimen

Plasma

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.