Sample Prep Set VMA, HVA, 5-HIAA with Collection Plates

Sample Prep Set VMA, HVA, 5-HIAA with Collection Plates

Order no.: 80800/96
96 Tests
  • 5-Hydroxyindoleacetic acid (5-HIAA)
  • Homovanillic acid (HVA)
  • Vanillylmandelic acid (VMA)

Simple and fast sample prep
Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls
Fully automated workflow solution available

Description

MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
With collection plates for 96 tests (80800/96) or 480 tests (80800/480), suitable for manual and automated sample preparation

 

Note: In addition to this Sample Prep Set the products of the Chromatographic Platform (Analytical Column, Mobile Phases A and B, Rinsing Solution) are required.

 

Clinical Relevance

This Sample Prep Set allows the quantitative determination of vanillylmandelic acid (VMA), homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA) in patient urine samples by LC-MS/MS. It is used for the diagnostics of neuroblastoma and carcinoid tumours.

 

Product advantages

  • Simple and fast sample prep consisting of 2 pipetting steps and subsequent injection
  • Isotopically labelled internal standard for each analyte
  • 6PLUS1® Multilevel Calibrator Set and MassCheck® controls ensure high precision of results
  • Fully automated workflow solution available

 

This Sample Prep Set encompasses all required components for an efficient and fast sample preparation.

 

A fully automated workflow solution is also available.

 

Alternatively, there is also a method available using autosampler vials (80800).

 

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Number of Tests

96

Parameter

5-Hydroxyindoleacetic acid (5-HIAA), Homovanillic acid (HVA), Vanillylmandelic acid (VMA)

Analysis Time

3 min

Limit of quantification

0.2 - 2.6 mg/l

Linearity

VMA, 5-HIAA up to 150 mg/l
HVA up to 250 mg/l

Recovery

95 - 113 %

Intraassay

CV 2.5 - 6.7 %

Interassay

CV 3.7 - 7.7 %

Column temperature

+25 °C

Specimen

24h urine or spontaneous urine

Pre-analytic Treatment

24 h urine is collected in a suitable container with 10 ml of 10–25 % HCl (VMA, HVA) or 10 ml glacial acetic acid (5-HIAA).
Spontaneous urine can be also analysed (see instruction manual).

Patient specimens are stable for up to 14 days at +2 to +8 °C in amber coloured vials.
For longer storage periods keep samples frozen below -18 °C.

Sample Preparation

  • Pipette 50 μl sample/calibrator/MassCheck® control into a well of the collection plate.
  • Add 25 μl Internal Standard Mix.
  • Add 425 μl Stabilisation Buffer.
  • Seal collection plate with adhesive seal.
  • Agitate collection plate for 2 min at 1200 rpm (orbit 2 mm) and transfer to autosampler.
  • Inject eluates into the LC-MS/MS system.

Sample Stability

The prepared samples are stable for up to 5 days (VMA and HVA) or up to 3 days ( 5-HIAA) at +2°C to +8°C in amber coloured vials. For longer storage periods keep samples frozen below -18°C.

Injection Volume

≤ 2 µl

Ionisation

ESI positive

MS/MS-Mode

MRM

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Calibrators

Calibrators available separately

Controls

Controls available separately