Sample Prep Set Total Metanephrines (free + conjugated) with 96 SPE Well Plate

Sample Prep Set Total Metanephrines (free + conjugated) with 96 SPE Well Plate

Order no.: 80700/96
96 Tests
  • 3-Methoxytyramine
  • Metanephrine
  • Normetanephrine

Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls
Fully automated workflow solution available

Description

MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With 96 SPE well plate for 96 analyses (80600/96), suitable for manual and automated sample prep procedure

 

Note: In addition to this Sample Prep Set the products of the Chromatographic Platform (Analytical Column, Mobile Phases A and B, Rinsing Solution) are required.

 

Clinical relevance

This Sample Prep Set allows the quantitative determination of total metanephrine, normetanephrine and 3-methoxytyramine in patient urine samples by LC-MS/MS.
One typical application for this kit is the analysis of patient samples with a suspicion of catecholamine-secreting tumours.

 

Product advantages

  • Isotopically labelled internal standard for each analyte
  • 6PLUS1® Multilevel Calibrator Set and MassCheck®controls ensure high precision of results.
  • Fully automated workflow solution available


This Sample Prep Set encompasses all required components for an efficient and fast sample preparation.

A fully automated workflow solution is also available.

Alternatively, there is also a method available using Sample Clean Up Columns (80700/C).

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Number of Tests

96

Parameter

3-Methoxytyramine, Metanephrine, Normetanephrine

Analysis Time

4 min

Linearity

Metanephrine, Normetanephrine up to 20 mg/l
3-Methoxytyramine up to 10 mg/l

Recovery

92–107 %

Intraassay

CV = 2.5–5.6 %

Interassay

CV = 3.2–4.9 %

Specimen

24h urine or spontaneous urine

Pre-analytic Treatment

24 h urine is collected in a suitable container with 10 ml of 10–25 % HCl.

Sample Preparation

  • Pipette 200 μl sample/calibrator/MassCheck® control into each well of the Hydrolysis Plate.
  • Add 50 μl Internal Standard Mix + 25 μl Hydrolysis Buffer.
  • Seal Hydrolysis Plate with pierceable adhesive seal and cover.
  • Hydrolyse in the oven for 45 min at 120 °C.
  • Cool for at least 10 min at +2 to +8 °C.
  • Place a 96 SPE Well Plate onto a Collection Plate.
  • Pipette 50 μl hydrolysate into each well of the 96 SPE Well Plate.
  • Add 600 μl Neutralisation Buffer.
  • Centrifuge 5 min at 150 x g, discard effluent.
  • Add 0.9 ml Wash Buffer 1, centrifuge 5 min at 150 x g, discard effluent.
  • Add 2 x 0.9 ml Wash Buffer 2, centrifuge each time 1 min at 150 x g, discard effluent.
  • Centrifuge 1 min at 2000 x g to dryness, discard effluent.
  • Place 96 SPE Well Plate onto a new Collection Plate.
  • Pipette 500 μl Elution Buffer into each well and centrifuge 2 min at 100 x g.
  • Pipette 500 μl Dilution Buffer into each well of the Collection Plate.
  • Seal with adhesive seal.
  • Shake for 2 min at 900 rpm, transfer to autosampler.
  • Inject up to 20 μl into the LC-MS/MS system.

Sample Stability

Samples are stable for at least 7 days at +2°C to +8°C in amber coloured vials. For longer storage periods keep samples frozen below -18°C.

Injection Volume

≤ 20 µl

Ionisation

ESI positive

MS/MS-Mode

MRM

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Hydrolysis Buffer


Order no.: 80703

Internal Standard Mix


Order no.: 80704

Neutralisation Buffer


Order no.: 80605

Wash Buffer 1


Order no.: 80606

Wash Buffer 2


Order no.: 80607

Elution Buffer


Order no.: 80608

Dilution Buffer


Order no.: 80609

Collection Plates


Order no.: 80056

96 SPE Well Plate


Order no.: 80057

Hydrolysis Plate


Order no.: 80058

Calibrators

Controls

Controls available separately