Sample Prep Set Catecholamines, free Metanephrines, Serotonin with Sample Clean Up Columns

Sample Prep Set Catecholamines, free Metanephrines, Serotonin with Sample Clean Up Columns

Order no.: 80600/C
96 Tests
  • 3-Methoxytyramine
  • Adrenaline (Epinephrine)
  • Dopamine
  • Metanephrine
  • Noradrenaline
  • Normetanephrine
  • Serotonin

7 Analytes with 1 Sample Prep Set
Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls

Description

MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With Sample Clean Up Columns, for manual sample prep procedure

 

Note: In addition to this Sample Prep Set the products of the Chromatographic Platform (Analytical Column, Mobile Phases A and B, Rinsing Solution) are required.

 

Clinical relevance

This Sample Prep Set allows the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine), dopamine, 3-methoxytyramine, metanephrine, normetanephrine and serotonin in patient urine samples by LC-MS/MS.
One typical application for this kit is the analysis of patient samples with suspected catecholamine or serotonin secreting tumours.

 

Product advantages

  • 7 analytes in one single run
  • Fast and efficient sample prep
    Correct pH, required for a precise analysis, is verified quickly and conveniently by a colour indicator and can be adjusted with a specific reagent, if necessary
  • Isotopically labelled internal standard for each analyte
  • 6PLUS1® Multilevel Calibrator Set and MassCheck® controls ensure high precision of results.


This Sample Prep Set encompasses all required components for an efficient and fast sample preparation.

There is also a method using 96 SPE well plates (80600/96 & 80600/480) as well as the corresponding fully automated workflow solution available for laboratories with a higher sample throughput.

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Number of Tests

96

Parameter

3-Methoxytyramine, Adrenaline (Epinephrine), Dopamine, Metanephrine, Noradrenaline, Normetanephrine, Serotonin

Analysis Time

4 min

Limit of quantification

1.0 - 13.5 μg/l

Linearity

1600 - 5000 µg/l

Intraassay

CV = 1.5 - 8.1 %

Interassay

CV = 3.1 - 10.7 %

Specimen

24 h urine or spontaneous urine

Pre-analytic Treatment

24 h urine collected in a suitable container with 10 ml of 10–25 % HCl.
Spontaneous urine can be also analysed (see instruction manual).

 

Patient specimens are stable for at least 7 days at +2 to +8 °C in amber coloured vials.
For longer storage periods keep samples frozen below -18 °C.

Sample Preparation

  • Pipette 200 µl of the sample/calibrator/MassCheck® control into each Sample Clean Up Column.
  • Add 50 µl Internal Standard Mix and 600 µl Neutralisation Buffer.
  • Check for correct colour.
  • Centrifuge 5 min at 150 x g, discard effluent.
  • Add 0.9 ml Wash Buffer 1 and centrifuge 5 min at 150 x g, discard effluent.
  • Add 2 x 0.9 ml Wash Buffer 2 and centrifuge each time 1 min at 150 x g, discard effluent.
  • Centrifuge 1 min at 2000 x g to dryness, discard effluent.
  • Put Sample Clean Up Columns on new glass collection vials (e.g. glass autosampler vials).
  • Add 500 µl Elution Buffer and centrifuge 2 min at 100 x g.
  • Add 500 µl Dilution Buffer to each glass vial.
  • Seal each glass vial.
  • Shortly vortex each glass vial and transfer vials to autosampler.
  • Inject up to 10 µl.

Sample Stability

The prepared samples are stable for at least 5 days at +2°C to +8°C in amber coloured vials. For longer storage periods keep samples frozen below -18°C.

Injection Volume

≤ 10 µl

Ionisation

ESI positive

MS/MS-Mode

MRM

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Calibrators

Controls