Sample Prep Set Catecholamines, free Metanephrines, Serotonin with 96 SPE Well Plate

Sample Prep Set Catecholamines, free Metanephrines, Serotonin with 96 SPE Well Plate

Order no.: 80600/96
96 Tests
  • 3-Methoxytyramine
  • Adrenaline (Epinephrine)
  • Dopamine
  • Metanephrine
  • Noradrenaline
  • Normetanephrine
  • Serotonin

7 Analytes with 1 Sample Prep Set
Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls
Fully automated workflow solution available

Description

MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With 96 SPE well plates for 96 analyses (80600/96) or 480 analyses (80600/480), suitable for manual and automated sample prep procedure

 

Note: In addition to this Sample Prep Set the products of the Chromatographic Platform (Analytical Column, Mobile Phases A and B, Rinsing Solution) are required.

 

Clinical relevance

This Sample Prep Set allows the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine), dopamine, 3-methoxytyramine, metanephrine, normetanephrine and serotonin in patient urine samples by LC-MS/MS.
One typical application for this kit is the analysis of patient samples with suspected catecholamine or serotonin secreting tumours.

 

Product advantages

  • 7 analytes in one single run
  • Fast and efficient sample prep
    Correct pH, required for a precise analysis, is verified quickly and conveniently by a colour indicator and can be adjusted with a specific reagent, if necessary
  • Isotopically labelled internal standard for each analyte
  • 6PLUS1® Multilevel Calibrator Set and MassCheck® controls ensure high precision of results.
  • Fully automated workflow solution available


This Sample Prep Set encompasses all required components for an efficient and fast sample preparation.

A fully automated workflow solution is also available.

Alternatively, there is also a method available using Sample Clean Up Columns (80600/C).

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Number of Tests

96

Parameter

3-Methoxytyramine, Adrenaline (Epinephrine), Dopamine, Metanephrine, Noradrenaline, Normetanephrine, Serotonin

Analysis Time

4 min

Limit of quantification

1 - 8 µg/l

Linearity

1600 - 5000 µg/l

Intraassay

CV 1.7 - 6.6 %

Interassay

CV 3.3 - 10.7 %

Specimen

24 h urine or spontaneous urine

Pre-analytic Treatment

24 h urine collected in a suitable container with 10 ml of 10–25 % HCl.
Spontaneous urine can be also analysed (see instruction manual).

 

Patient specimens are stable for at least 7 days at +2 to +8 °C in amber coloured vials.
For longer storage periods keep samples frozen below -18 °C.

Sample Preparation

  • Put 96 SPE Well Plate onto a Collection Plate.
  • Pipette 200 µl of the sample/calibrator/MassCheck® control into each well of the 96 SPE Well Plate.
  • Add 50 µl Internal Standard Mix and 600 µl Neutralisation Buffer.
  • Check for correct colour.
  • Centrifuge 5 min at 150 x g, discard effluent.
  • Add 0.9 ml Wash Buffer 1 and centrifuge 5 min at 150 x g, discard effluent.
  • Add 2 x 0.9 ml Wash Buffer 2 and centrifuge each time 1 min at 150 x g, discard effluent
  • Centrifuge 1 min at 2000 x g to dryness, discard effluent.
  • Place 96 SPE Well Plate onto a new Collection Plate.
  • Add 500 µl Elution Buffer to each well and centrifuge 2 min at 100 x g.
  • Add 500 µl Dilution Buffer to each well of the Collection Plate.
  • Seal Collection Plate with adhesive seal.
  • Shake for 2 min at 900 rpm and transfer to autosampler.
  • Inject up to 10 µl.

Sample Stability

The prepared samples are stable for at least 5 days at +2°C to +8°C in amber coloured vials. For longer storage periods keep samples frozen below -18°C.

Injection Volume

≤ 10 µl

Ionisation

ESI positive

MS/MS-Mode

MRM

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Internal Standard Mix


Order no.: 80604

Neutralisation Buffer


Order no.: 80605

Wash Buffer 1


Order no.: 80606

Wash Buffer 2


Order no.: 80607

Elution Buffer


Order no.: 80608

Dilution Buffer


Order no.: 80609

Collection Plates


Order no.: 80056

96 SPE Well Plate


Order no.: 80057

Calibrators

Controls