MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS

MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS

Order no.: 69000/RUO
400 Tests
  • Ethyl glucuronide (EtG)
  • Ethyl sulfate (EtS)

Very high column stability
Efficient one step sample prep
Robust, reliable & precise determination

Description

This MassChrom® kit from Chromsystems allows the quantitative determination of ethyl glucuronide (EtG) and ethyl sulfate (EtS) in urine with robustness and precision. The assay enables the analysis of both parameters with one sample preparation and one single injection with an analysis time of 6 minutes. The straightforward and efficient sample preparation consists of a single pipetting step that includes the instrument-specific dilution. 6PLUS1® Multilevel Calibrator Set and MassCheck® controls are also available separately.

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Number of Tests

400

Parameter

Ethyl glucuronide (EtG), Ethyl sulfate (EtS)

Analysis Time

6 min

Gradient

0.00–3.00 min, 0 % Mobile Phase B
3.01–5.50 min, 100 % Mobile Phase B
5.51–6.00 min, 0 % Mobile Phase B

Flow rate

0.5-1.0 ml/min

Specimen

Urine

Sample Preparation

Place 50 µl sample/calibrator/MassCheck® control in autosampler vial.
Dilute as required by the instrument with Master Mixture (minimum dilution of 1:5).
Vortex briefly.
Inject up to 20 µl into the LC-MS/MS system.

Master Mixture
Example for 100 research samples and a target dilution of 1:10: Add 2.5 ml Internal Standard Mix to 42.5 ml Dilution Buffer. Add 450 µl of this mixture to 50 µl sample.

Sample Stability

Research samples are stable up to 5 days at +2 to +8°C. For longer storage periods (max. 3 months), keep research samples frozen below -18°C.

Injection Volume

up to 20 µl

Ionisation

ESI (negative)

MS/MS-Mode

MRM

Additional Info

It is recommended to set the scan time to a value that allows a minimum of 10 data points over the whole peak width.

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Mobile Phase A


Order no.: 69001/RUO

Mobile Phase B


Order no.: 69002/RUO

Internal Standard Mix


Order no.: 69004/RUO

Dilution Buffer


Order no.: 69005/RUO

Rinsing Solution


Order no.: 69009/RUO

Calibrators

Calibrators available separately

Controls