0040 0050 HPLC endocrine urine controls

Endocrine Urine Controls

   
  • 3,4-Dihydroxymandelic acid (DOMA)
  • 3,4-Dihydroxyphenylacetic acid (DOPAC)
  • 3,4-Dihydroxyphenylalanine (L-DOPA)
  • 3,4-Dihydroxyphenylglycol (DHPG)
  • 3-Methoxy-4-hydroxyphenylglycol (MHPG)
  • 3-Methoxytyramine
  • 5-Hydroxyindoleacetic acid (5-HIAA)
  • Adrenaline (Epinephrine)
  • Dopamine
  • Homovanillic acid (HVA)
  • Metanephrine
  • Noradrenaline
  • Normetanephrine
  • Serotonin
  • Vanillylmandelic acid (VMA)

Order no.: 0040/0050

Product Name Qty
Endocrine Urine Control, normal range
Order no.: 0040
Endocrine Urine Control, pathological range
Order no.: 0050

Description

Endocrine Urine Controls, lyophilised

0040 Endocrine Urine Control, Normal Range (lyoph.), 10 x 8 ml
0050 Endocrine Urine Control, Pathological Range (lyoph.), 10 x 8 ml

These lyophilised quality controls from Chromsystems are based on human urine and should be used according to the instruction manual. They are designed to monitor the accuracy and precision of analytical procedures in clinical diagnostics for the quantitative determination of endocrine hormones and their metabolites in urine. Further values are related to parameters for neurochemical analyses. While following the instructions the controls should be handled and measured in the same manner as a patient specimen.

Technical Data

Method of Analysis

HPLC

Parameter

3,4-Dihydroxymandelic acid (DOMA), 3,4-Dihydroxyphenylacetic acid (DOPAC), 3,4-Dihydroxyphenylalanine (L-DOPA), 3,4-Dihydroxyphenylglycol (DHPG), 3-Methoxy-4-hydroxyphenylglycol (MHPG), 3-Methoxytyramine, 5-Hydroxyindoleacetic acid (5-HIAA), Adrenaline (Epinephrine), Dopamine, Homovanillic acid (HVA), Metanephrine, Noradrenaline, Normetanephrine, Serotonin, Vanillylmandelic acid (VMA)

Concentrations

Please note: Concentration values shown here are typical. For lot specific concentration values please refer to the product insert supplied with the materials. The product inserts can also be requested from us directly or downloaded from our website after login.

Endocrine Urine Control Normale range Pathological range
AnalyteMethodUnitValueRangeValueRange
Noradrenaline HPLC g/l
nmol/l
69.6
411
55.7-83.5
329-493
206
1216
165-247
972-1459
Adrenaline HPLC g/l
nmol/l
13.8
75.4
11.1-16.6
60.3-90.5
47.9
261
38.3-57.4
209-314
Dopamine HPLC g/l
nmol/l
207
1352
166-249
1082-1623
435
2840
348-522
2272-3408
Serotonine HPLC g/l
nmol/l
164
932
132-197
746-1119
381
2160
305-457
1728-2592
Normetanephrine HPLC g/l
nmol/l
313
1709
250-376
1367-2051
992
5414
793-1190
4331-6496
Metanephrine HPLC g/l
nmol/l
162
819
129-194
655-983
363
1840
290-436
1472-2208
3-Methoxytyramine HPLC g/l
nmol/l
156
930
117-194
698-1163
349
2084
261-436
1563-2606
Vanillylmandelic acid (VMA) HPLC mg/l
µmol/l
5.07
25.6
4.06-6.08
20.4-30.7
15.3
77.3
12.3-18.4
61.8-92.7
5-Hydroxyindole-3-acetic acid (5-HIAA) HPLC mg/l
µmol/l
4.75
24.8
3.80-5.70
19.9-29.8
11.9
62.5
9.55-14.3
50.0-74.9
Homovanillic acid (HVA) HPLC mg/l
µmol/l
5.23
28.7
4.18-6.28
23.0-34.5
13.2
72.2
10.5-15.8
57.8-86.6
3,4-Dihydroxyphenylacetic acid (DOPAC) spiked value mg/l
µmol/l
3.00
17.8
2.40-3.60
14.2-21.4
10.0
59.3
7.99-12.0
47.5-71.2
3-Methoxy-4-hydroxyphenylglycol (MHPG) spiked value mg/l
µmol/l
1.20
6.49
0.96-1.44
5.19-7.79
3.79
20.5
3.03-4.55
16.4-24.7
3,4-Dihydroxyphenylglycol (DHPG) spiked value mg/l
µmol/l
0.60
3.53
0.48-0.72
2.82-4.23
2.60
15.3
2.08-3.12
12.2-18.3
3,4-Dihydroxymandelic acid (DOMA) spiked value mg/l
µmol/l
0.40
85.5
0.32-0.48
1.72-2.58
3.59
85.5
2.87-4.31
15.6-23.4
3,4-Dihydroxyphenylalanine (L-DOPA) spiked value mg/l
µmol/l
85.1
431
68.1-102
345-518
250
1268
200-300
1014-1521

Reconstitution

Carefully remove the metal seal and rubber stopper from the vials. Add exactly 8.0 ml of distilled water into each vial. Return the stopper and incubate for 10 to 15 minutes at room temperature. Swirl the vials to dissolve the contents until homogeneity.

Storage conditions

Stored at +2 to +8 °C and unopened, the lyophilised controls are stable until the expiry date specified on the vial labels. The reconstituted controls can be stored up to 5 days light protected and tightly capped at +2 to +8 C. Since the different parameters of these urine controls have different stability characteristics in solution, the specific storage conditions for the individual parameters should also be selected for the controls. If the controls cannot be used within this period, they should be aliquoted and stored frozen below -18 °C (up to 3 months). For more details about storage conditions please check your instruction manual.