0010 0020 HPLC endocrine plasma controls

Endocrine Plasma Controls

   
  • 3,4-Dihydroxymandelic acid (DOMA)
  • 3,4-Dihydroxyphenylacetic acid (DOPAC)
  • 3,4-Dihydroxyphenylalanine (L-DOPA)
  • 3,4-Dihydroxyphenylglycol (DHPG)
  • 3-Methoxy-4-hydroxyphenylglycol (MHPG)
  • 3-Methoxytyramine
  • 5-Hydroxyindoleacetic acid (5-HIAA)
  • Adrenaline (Epinephrine)
  • Dopamine
  • Homovanillic acid (HVA)
  • Metanephrine
  • Noradrenaline
  • Normetanephrine
  • Serotonin
  • Vanillylmandelic acid (VMA)

Order no.: 0010/0020

Product Name Qty
Endocrine Plasma Control, normal range
Order no.: 0010
Endocrine Plasma Control, pathological range
Order no.: 0020

Description

Endocrine Plasma Controls, lyophilised

0010 Endocrine Plasma Control, Normal Range (lyoph.), 10 x 5 ml
0020 Endocrine Plasma Control, Pathological Range (lyoph.), 10 x 5 ml

These lyophilised quality controls from Chromsystems are based on human plasma and should be used according to the instruction manual. They are designed to monitor the accuracy and precision of analytical procedures in clinical diagnostics for the quantitative determination of endocrine hormones and their metabolites in plasma. Further values are related to parameters for neurochemical analyses. While following the instructions the controls should be handled and measured in the same manner as a patient specimen.

Technical Data

Method of Analysis

HPLC

Parameter

3,4-Dihydroxymandelic acid (DOMA), 3,4-Dihydroxyphenylacetic acid (DOPAC), 3,4-Dihydroxyphenylalanine (L-DOPA), 3,4-Dihydroxyphenylglycol (DHPG), 3-Methoxy-4-hydroxyphenylglycol (MHPG), 3-Methoxytyramine, 5-Hydroxyindoleacetic acid (5-HIAA), Adrenaline (Epinephrine), Dopamine, Homovanillic acid (HVA), Metanephrine, Noradrenaline, Normetanephrine, Serotonin, Vanillylmandelic acid (VMA)

Concentrations

Please note: Concentration values shown here are typical. For lot specific concentration values please refer to the product insert supplied with the materials. The product inserts can also be requested from us directly or downloaded from our website after login.

SubstanceMethodUnitValueRangeValueRange
      Normal Range Pathological Range
Catecholamines
Noradrenaline HPLC ng/l
pmol/l
269
1587
188-349
1111-2063
2012
11892
1509-2515
8919-14865
Adrenaline HPLC ng/l
pmol/l
887.5
478
61.3-114
334-621
477
2605
358-596
1953-3256
Dopamine HPLC ng/l
pmol/l
176
1150
123-229
805-1495
848
5536
593-1102
3875-7197
Serotonin HPLC g/l
nmol/l
146
829
117-175
663-994
347
1968
278-417
1574-2362
Metanephrines
Metanephrine spiked value ng/l
pmol/l
60
304
48-72
243-365
1500
7605
1200-1800
6084-9126
Normetanephrine spiked value ng/l
pmol/l
100
546
80-120
437-655
7000
38220
5600-8400
30576-45864
3-Methoxytyramine spiked value       1500
8970
1200-1800
7176-10764
Vanillylmandelic acid
(VMA)
spiked value g/l
nmol/l
7.5
37.8
6.0-9.0
30.2-45.4
80.1
403
64.0-96.0
323-484
Homovanillic acid
(HVA)
spiked value g/l
nmol/l
10
54.9
8-12
43.9-65.9
100,0
549
80.1-120.0
440-659
5-Hydroxyindole-3-acetic acid
(5-HIAA)
spiked value g/l
nmol/l
8.0
41.8
6.4-9.6
33.5-50.2
40.0
209
32.0-48.0
167-251

3,4-Dihydroxyphenyl-acetic acid (DOPAC)

spiked value g/l
nmol/l
4.0
23.8
3.2-4.8
19.0-28.5
55.0
326
44.0-66.0
261-392
3-Methoxy-4-hydroxy-phenylglycol (MHPG) spiked value g/l
nmol/l
5.0
27.1
4.0-6.0
21.7-32.5
30.0
163
24.0-36.0
130-195
3,4-Dihydroxyphenyl-glycol (DHPG) spiked value g/l
nmol/l
3.0
17.6
2.4-3.6
14.1-21.2
15.0
88.2
12.0-18.0
70.6-106
3,4-Dihydroxymandelic acid (DOMA) spiked value g/l
nmol/l
0.80
4.34
0.64-0.96
3.5-5.2
5.0
27.2
4.0-6.0
21.7-32.6
3,4-Dihydroxyphenyl-alanine (L-DOPA) spiked value g/l
nmol/l
1.5
7.61
1.2-1.8
6.08-9.13
6.0
30.4
4.8-7.2
24.3-36.5

Reconstitution

Carefully remove the metal seal and rubber stopper from the vials. Add exactly 5.0 ml of distilled water into each vial. Return the stopper and incubate for 10 to 15 minutes at room temperature. Swirl the vials to dissolve the contents until homogeneity.

Storage conditions

Stored at +2 to +8 °C and unopened, the lyophilised controls are stable until the expiry date specified on the vial labels. The reconstituted controls can be stored up to 2 days light protected and tightly capped at +2 to +8 C. The reconstituted controls have the same stability as a fresh plasma specimen. Since the different parameters of these plasma controls have different stability characteristics in solution, the specific storage conditions for the individual parameters should also be selected for the controls. If the controls cannot be used within this period, they should be aliquoted and stored frozen below -18 °C (up to 2 months). For more details about storage conditions please check your instruction manual.

Your Feedback is Appreciated

* Required Fields