6000-COMBI HPLC Kit Catecholamines Urine combined analysis

Catecholamines in Urine, Combined analysis - HPLC

Order no.: 6000/COMBI
100 Tests
  • Adrenaline (Epinephrine)
  • Dopamine
  • Noradrenaline

Short analysis time < 6 min
Only 1 HPLC column and 1 Mobile Phase for 2020/COMBI and 6000/COMBI

Description

Clinical relevance

The catecholamines adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are biogenic amines, which play a central role in the body as hormones and neurotransmitters. Adrenaline and noradrenaline are produced in the adrenal medulla and the sympathetic nervous system. Dopamine is a neurotransmitter in the brain and acts as a local messenger substance in the peripheral nervous system.

 

Product advantages

  • Short analysis time of less than 6 minutes
  • No changes to setup are needed due to identical column and mobile phase

 

This kit enables the combined determination of catecholamines together with metanephrine in urine (Kit 2020/COMBI). It is an exceptionally sensitive and reliable method for detecting pheochromocytomas with an analysis time of less than 6 minutes. The highly selective separation column and the optimised mobile phase are used for the analysis of both catecholamines and metanephrines, so no change of the chromatographic system is required. The established specific sample preparation using solid phase extraction guarantees simple and safe handling and reproducible results.

Chromatogram

Technical Data

Method of Analysis

HPLC

Number of Tests

100

Parameter

Adrenaline (Epinephrine), Dopamine, Noradrenaline

Analysis Time

< 6 min

Limit of quantification

1 μg/l

Linearity

1–1000 μg/l

Recovery

81-88 %

Intraassay

CV = 0.9-2.7 %

Interassay

CV = 2.1-4.1 %

Column temperature

30 °C

Flow rate

0.9 ml/min

Specimen

Urine

Sample Preparation

Stabilisation

  • 3 ml urine + 100 μl Internal Standard + 6 ml Neutralisation Buffer.
  • Adjust pH value.

Extraction

  • Apply the sample on the Sample Clean Up Column, discard effluent.
  • Fill up twice with ultrapure water (HPLC grade) and discard the effluent.

Elution

  • Apply 6 ml Elution Buffer on the Sample Clean Up Column and collect the eluate.
  • Add 200 μl Stabilisation Reagent.
    Mix each eluate thoroughly.

HPLC Analysis

  • Inject 10 μl into the HPLC system.

Injection Volume

10 µl

Additional Info

For the combined HPLC analysis of catecholamines and metanephrines any isocratic HPLC system with electrochemical detector can be used.

Potential

+580/+640 mV

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Mobile Phase


Order no.: 2031/COMBI

Mobile Phase, 10 x 1000 ml


Order no.: 2032/COMBI

Internal Standard


Order no.: 6004

Neutralisation Buffer


Order no.: 6055

Elution Buffer


Order no.: 6006

Controls

Controls available separately