6000-COMBI HPLC Kit Catecholamines Urine combined analysis

Catecholamines in Urine, Combined analysis - HPLC

Order no.: 6000/COMBI
100 Tests
  • Adrenaline (Epinephrine)
  • Dopamine
  • Noradrenaline

Short analysis time < 6 min
Only 1 HPLC column and 1 Mobile Phase for 2020/COMBI and 6000/COMBI


Clinical relevance

The catecholamines adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are biogenic amines, which play a central role in the body as hormones and neurotransmitters. Adrenaline and noradrenaline are produced in the adrenal medulla and the sympathetic nervous system. Dopamine is a neurotransmitter in the brain and acts as a local messenger substance in the peripheral nervous system.


Product advantages

  • Short analysis time of less than 6 minutes
  • No changes to setup are needed due to identical column and mobile phase


This kit enables the combined determination of catecholamines together with metanephrine in urine (Kit 2020/COMBI). It is an exceptionally sensitive and reliable method for detecting pheochromocytomas with an analysis time of less than 6 minutes. The highly selective separation column and the optimised mobile phase are used for the analysis of both catecholamines and metanephrines, so no change of the chromatographic system is required. The established specific sample preparation using solid phase extraction guarantees simple and safe handling and reproducible results.


Technical Data

Method of Analysis


Number of Tests



Adrenaline (Epinephrine), Dopamine, Noradrenaline

Analysis Time

< 6 min

Limit of quantification

1 μg/l


1–1000 μg/l


81-88 %


CV = 0.9-2.7 %


CV = 2.1-4.1 %



Sample Preparation


  • 3 ml urine + 100 μl Internal Standard + 6 ml Neutralisation Buffer.
  • Adjust pH value.


  • Apply the sample on the Sample Clean Up Column, discard effluent.
  • Fill up twice with ultrapure water (HPLC grade) and discard the effluent.


  • Apply 6 ml Elution Buffer on the Sample Clean Up Column and collect the eluate.
  • Add 200 μl Stabilisation Reagent.
    Mix each eluate thoroughly.

HPLC Analysis

  • Inject 10 μl into the HPLC system.

Additional Info

For the combined HPLC analysis of catecholamines and metanephrines any isocratic HPLC system with electrochemical detector can be used.

Injection Volume

10 µl

Flow rate

0.9 ml/min

Column temperature

30 °C


+580/+640 mV

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Mobile Phase

Order no.: 2031/COMBI

Mobile Phase, 10 x 1000 ml

Order no.: 2032/COMBI

Internal Standard

Order no.: 6004

Neutralisation Buffer

Order no.: 6055

Elution Buffer

Order no.: 6006


Calibrators available separately


Controls available separately