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Description
The MassTox® Series A reagent kit allows the rapid quantitative determination of a multitude of drugs in serum or plasma by LC-MS/MS. To perform the analysis, you will need the MassTox® BASIC Kit A, the substance-specific MassTox® PARAMETER Set and the MassTox® MasterColumn® Series A.
Preparation of the research samples is the same for all parameters, based on a simple, effective protein precipitation process. The use of stable isotope-labelled (deuterated) internal standards ensures high performance of the analysis.
Content:
1 x Mobile Phase 1 (92001/RUO)
1 x Mobile Phase 2 (92002/RUO)
1 x Precipitation Reagent (92003/RUO)
1 x Extraction Buffer (92005/RUO)
1 x Dilution Buffer 1 (92007/RUO)
1 x Dilution Buffer 2 (92008/RUO)
1 x Rinsing Solution (92009/RUO)
2 x Reaction vials (33006/RUO)
Technical Data
Method of Analysis
LC-MS/MS
Number of Tests
200
Sample Preparation
The sample preparation is standard for all Series A analytes.
- Add 800 µl Internal Standard mix to 12 ml Precipitation Reagent to form mixture A
- Place 50 µl sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
- Add 25 µl Extraction Buffer
- Mix and incubate 2 min at ambient temperature (do not centrifuge)
- Add 250 µl of mixture A and mix 30 sec minimum (vortex)
- Centrifuge 5 min at 9000 x g
- Dilute the supernatant with Dilution Buffer prior to injection depending on instrument sensitivity
Please note
The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
