Antiepileptic Drugs in Serum/Plasma, HIGH RESOLUTION - HPLC

Antiepileptic Drugs in Serum/Plasma, HIGH RESOLUTION - HPLC

Order no.: 22000/HR/RUO
100 Tests
  • 10-OH-carbamazepine
  • Carbamazepine
  • Carbamazepine-10,11-epoxide
  • Ethosuximide
  • Lamotrigine
  • Oxcarbamazepine (Trileptal®)
  • Phenobarbital
  • Phenytoin
  • Primidone
  • Sultiame
  • Zonisamide

All relevant drugs in one run
Fast sample preparation

Description

This Chromsystems reagent kit is designed for the specific determination of the antiepileptic drugs ethosuximide, zonisamide, primidone, phenobarbital, phenytoin, carbamazepine, lamotrigine, trileptal® (oxcarbazepine, syn. oxcarbamazepine) and sultiame in serum or plasma. In addition, the analogues carbamazepine-10,11-epoxide, 10,11-dihydro-10,11-dihydroxycarbamazepine, 10-OH-carbamazepine and 2-ethyl-2-phenylmalondiamide (PEMA) can be analysed.
Sample preparation is based on a simple but efficient precipitation step. To separate and quantify all analytes a chromatography with high resolution is used.

Chromatogram

Technical Data

Method of Analysis

HPLC

Number of Tests

100

Parameter

10-OH-carbamazepine, Carbamazepine, Carbamazepine-10,11-epoxide, Ethosuximide, Lamotrigine, Oxcarbamazepine (Trileptal®), Phenobarbital, Phenytoin, Primidone, Sultiame, Zonisamide

Analysis Time

High Resolution: 22 min

Information

A precolumn has to be used.

Column temperature

ambient (~25 °C)

Flow rate

High Resolution: 1.2 ml/min

Specimen

Serum/Plasma

Sample Preparation

  • Pipette 100 μl serum/plasma (standards, controls, research specimens) and 150 μl Internal Standard into a labelled reaction vial, mix briefly (vortex).
  • Add 50 μl Precipitation Reagent, mix 1 min (vortex).
  • Centrifuge 10–15 min at 9000 x g.
  • Mix 100 μl of the supernatant with 100 μl Stabilisation Buffer.
  • Inject 20 μl into the HPLC system.

Sample Stability

The research samples can be kept refrigerated (+2 to +8°C) for approx. one week. For longer storage (up to 2 weeks) deep-freeze them below –18°C.

Injection Volume

20 µl

Additional Info

Analysis of antiepileptic drugs requires a simple isocratic HPLC system with HPLC pump, injector and UV detector. The use of a thermostat-controlled column oven avoids temperature variations and optimises the stability of the separation.

Wavelength

204 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Mobile Phase, High Resolution


Order no.: 22001/HR/RUO

Precipitation Reagent


Order no.: 22003/RUO

Internal Standard


Order no.: 22004/RUO

Stabilisation Buffer


Order no.: 22006/RUO

Reaction vials


Order no.: 3006/RUO

Calibrators

Calibrators available separately

Controls

Controls available separately

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