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Description
6PLUS1® Multilevel Serum Calibrator Set Steroid Panel 2, lyophilised
These lyophilised calibrators from Chromsystems are based on human serum and should be used according to the instruction manual. They are designed to calibrate the measurement method in clinical diagnostics for the quantitative determination of steroids/Panel 2 in serum/plasma. While following the instructions the calibrators should be handled and measured in the same manner as a patient specimen.
These calibrators are only available as multilevel calibrator set with 6 different concentration levels plus 1 blank value.
Technical Data
Method of Analysis
LC-MS/MS
Content
7 x 3 ml (lyoph.)
Parameter
11-Deoxycorticosterone, 17-OH-progesterone, Androstenedione, Dehydroepiandrosterone (DHEA), Dehydroepiandrosterone sulfate (DHEA-S), Dihydrotestosterone (DHT), Estradiol, Progesterone, Testosterone
Concentrations
Please note: Concentration values shown here are typical. For lot specific concentration values please refer to the product insert supplied with the materials. The product inserts can also be requested from us directly or downloaded from our website after login.
| Substance | Unit | Calibrator 1 | Calibrator 2 | Calibrator 3 | Calibrator 4 | Calibrator 5 | Calibrator 6 | Blank Calibrator |
|---|---|---|---|---|---|---|---|---|
| Androstenedione | µg/l | 0.202 | 0.408 | 0.820 | 1.54 | 5.09 | 15.3 | n.d. (< 0.020) |
| 11-Deoxycorticosterone | µg/l | 0.049 | 0.098 | 0.146 | 0.288 | 0.721 | 2.80 | n.d. (< 0.023) |
| DHEA | µg/l | 1.04 | 5.25 | 10.3 | 15.6 | 30.7 | 60.7 | n.d. (< 0.229) |
| DHEAS | µg/l | 117 | 558 | 1097 | 2066 | 3949 | 5887 | n.d. (< 24.4) |
| Dihydrotestosterone | µg/l | 0.061 | 0.111 | 0.257 | 0.501 | 0.942 | 1.50 | n.d. (< 0.042) |
| Estradiol | µg/l | 0.043 | 0.105 | 0.260 | 0.530 | 1.59 |
5.33 |
n.d. (< 0.026) |
| 17-Hydroxyprogesterone | µg/l | 0.097 | 0.498 | 1.01 | 2.00 | 3.98 | 15.0 | n.d. (< 0.040) |
| Progesterone | µg/l | 0.180 | 0.805 | 2.11 | 5.27 | 10.3 | 26.0 | n.d. (< 0.030) |
| Testosterone | µg/l | 0.055 | 0.250 | 0.982 | 2.94 | 5.82 | 11.6 | n.d. (< 0.005) |
n.d. = not determined (< LOQ)
Reconstitution
Carefully remove the metal seal and rubber stopper from the vials. Add exactly 3.0 ml of distilled water into each vial. Return the stopper and incubate for 10 to 15 minutes at room temperature. Swirl the vials to dissolve the contents until homogeneity.
Storage conditions
Stored below -18 °C and unopened, the lyophilised calibrators are stable until the expiry date specified on the vial labels. The reconstituted calibrators can be stored up to 10 days light protected and tightly capped at +2 to +8 C. If the calibrators cannot be used within this period, they should be aliquoted and stored frozen below -18 °C (up to 3 months). For more details about storage conditions please check your instruction manual.
