6PLUS1® Multilevel Serum Calibrator Set MassChrom® Steroid Panel 2

6PLUS1® Multilevel Serum Calibrator Set MassChrom® Steroid Panel 2

Order no.: 72039/RUO
  • 11-Deoxycorticosterone
  • 17-OH-progesterone
  • Androstenedione
  • Dehydroepiandrosterone (DHEA)
  • Dehydroepiandrosterone sulfate (DHEA-S)
  • Dihydrotestosterone (DHT)
  • Estradiol
  • Progesterone
  • Testosterone

MassChrom® Steroids in Serum/Plasma - LC-MS/MS

Description

6PLUS1® Multilevel Serum Calibrator Set MassChrom® Steroid Panel 2, lyophilised
For MassChrom® Steroids in Serum/Plasma with Sample Clean Up Columns or 96 SPE Well Filter Plates.

These lyophilised calibrators from Chromsystems are based on human serum and should be used according to the instruction manual. They are designed to calibrate the measurement method in clinical diagnostics for the quantitative determination of steroids/Panel 2. While following the instructions the calibrators should be handled and measured in the same manner as research samples.
These calibrators are only available as multilevel calibrator set with 6 different concentration levels plus 1 blank value.

 

Technical Data

Method of Analysis

LC-MS/MS

Content

7 x 3 ml (lyoph.)

Parameter

11-Deoxycorticosterone, 17-OH-progesterone, Androstenedione, Dehydroepiandrosterone (DHEA), Dehydroepiandrosterone sulfate (DHEA-S), Dihydrotestosterone (DHT), Estradiol, Progesterone, Testosterone

Concentrations

Please note: Concentration values shown here are typical. For lot specific concentration values please refer to the product insert supplied with the materials. The product inserts can also be requested from us directly. 

Substance UnitCalibrator 1Calibrator 2Calibrator 3Calibrator 4Calibrator 5Calibrator 6Blank Calibrator
Androstenedione µg/l 0.202 0.408 0.820 1.54 5.09 15.3 n.d. (< 0.020)
11-Deoxycorticosterone µg/l 0.049 0.098 0.146 0.288 0.721 2.80 n.d. (< 0.023)
DHEA µg/l 1.04 5.25 10.3 15.6 30.7 60.7 n.d. (< 0.229)
DHEAS µg/l 117 558 1097 2066 3949 5887 n.d. (< 24.4)
Dihydrotestosterone µg/l 0.061 0.111 0.257 0.501 0.942 1.50 n.d. (< 0.042)
Estradiol µg/l 0.043 0.105 0.260 0.530 1.59

5.33

n.d. (< 0.026)
17-Hydroxyprogesterone µg/l 0.097 0.498 1.01 2.00 3.98 15.0 n.d. (< 0.040)
Progesterone µg/l 0.180 0.805 2.11 5.27 10.3 26.0 n.d. (< 0.030)
Testosterone µg/l 0.055 0.250 0.982 2.94 5.82 11.6 n.d. (< 0.005)
n.d. = not determined (< LOQ)

Reconstitution

Carefully remove the metal seal and rubber stopper from the vials. (Caution when removing the metal seal: risk of injuries due to sharp cutting edges!). Add exactly 3.0 ml of distilled water into each vial. Return the stopper and incubate for 10 to 15 minutes at room temperature. Swirl the vials to dissolve the contents until homogeneity.

Storage conditions

Stored below -18 °C and unopened, the lyophilised calibrators are stable until the expiry date specified on the vial labels. The reconstituted calibrators can be stored up to 10 days light protected and tightly capped at +2 to +8 C. If the calibrators cannot be used within this period, they should be aliquoted and stored frozen below -18 °C (up to 3 months). For more details about storage conditions please check your instruction manual.