6PLUS1® Multilevel Plasma Calibrator Set Anti-HIV Drugs

6PLUS1® Multilevel Plasma Calibrator Set Anti-HIV Drugs

Order no.: 92053/RUO
  • Amprenavir
  • Atazanavir
  • Darunavir
  • Delavirdine
  • Efavirenz
  • Elvitegravir
  • Etravirine
  • Indinavir
  • Lopinavir
  • Maraviroc
  • Nelfinavir
  • Nelfinavir-M8
  • Nevirapine
  • Raltegravir
  • Rilpivirine
  • Ritonavir
  • Saquinavir
  • Tipranavir

Component of the Parameter Set Anti-HIV Drugs

Description

6PLUS1® Multilevel Calibrator Set for the Parameter Set Anti-HIV Drugs in serum/plasma, lyophilised

The calibrator is based on human plasma and is suggested to be used according to the instructions accompanying the assay procedure being used. While following these instructions the calibrator is to be handled in the same manner as the research sample(s).

 

6PLUS1® Multilevel Plasma Calibrator Set for the Parameter Set Anti-HIV Drugs, lyophilised.

These lyophilised calibrators from Chromsystems are based on human plasma and should be used according to the instruction manual. They are designed to calibrate the measurement method for the quantitative determination of anti-HIV drugs. While following the instructions the calibrators should be handled and measured in the same manner as research samples.
These calibrators are only available as multilevel calibrator set with 6 different concentration levels plus 1 blank value.

Technical Data

Method of Analysis

LC-MS/MS

Content

7 x 1 ml (lyoph.)

Parameter

Amprenavir, Atazanavir, Darunavir, Delavirdine, Efavirenz, Elvitegravir, Etravirine, Indinavir, Lopinavir, Maraviroc, Nelfinavir, Nelfinavir-M8, Nevirapine, Raltegravir, Rilpivirine, Ritonavir, Saquinavir, Tipranavir

Concentrations

Please note: Concentration values shown here are typical. For lot specific concentration values please refer to the product insert supplied with the materials. The product inserts can also be requested from us directly.

SubstanceCalibrator 1
(µg/l)
Calibrator 2
(µg/l)
Calibrator 3
(µg/l)
Calibrator 4
(µg/l)
Calibrator 5
(µg/l)
Calibrator 6
(µg/l)
Blank Calibrator
(µg/l)
Amprenavir 203 729 1239 1772 2272 2793 n.d. (< 10.0)
Darunavir 48.4 1159 2277 3359 4475 5531 n.d. (< 12.0)
Delavirdine 683 3060 5294 7510 9897 11793 n.d. (< 6.0)
Maraviroc 26.9 289 547 807 1071 1315 n.d. (< 2.0)
Nevirapine 1941 3067 4168 5387 6482 7653 n.d. (< 20)
Raltegravir 27.6 158 282 407 537 663 n.d. (< 7.0)
Efavirenz 638 1463 2283 3138 3962 4771 n.d. (< 65)
Indinavir 7.86  751 1495  2197  2894 3533 n.d. (< 2.0)
Nelfinavir 541 1023 1504 1978  2437 2875 n.d. (< 2.0)
M8-Nelfinavir 164 514 850 1204 1536 1855 n.d. (< 1.0)
Rilpivirine 37.2 115 187 260 333 403 n.d. (< 5.0)
Saquinavir 35.0 222 404 590 774 954 n.d. (< 3.0)
Atazanavir 60.2 733 1379 2049 2652 3324 n.d. (< 3.0)
Elvitegravir 135 350 571 793 1015 1238 n.d. (< 10)
Etravirine 55.5 639 1211 1794 2347 2931 n.d. (< 20) 
Lopinavir 733 2943 5073 7224 9236 11161 n.d. (< 4.0) 
Ritonavir 187 2970 5610 8172 10470 12587 n.d. (< 8.0) 
Tipranavir 7985 17259 26275 35608 44118 52906 n.d. (< 40) 

n.d. = not determined
The blank level is manufactured using the same matrix pool as used for the calibrator levels 1-6. It was not fortified with any anti-HIV drugs.

Reconstitution

Carefully remove the metal seal and rubber stopper from the vials. (Caution when removing the metal seal: risk of injuries due to sharp cutting edges!). Add exactly 1.0 ml of distilled water into each vial. Return the stopper and incubate for 10 to 15 minutes at room temperature. Swirl the vials to dissolve the contents until homogeneity.

Storage conditions

Stored below -18 °C and unopened, the lyophilised calibrators are stable until the expiry date specified on the vial labels. The reconstituted calibrators can be stored in glass containers up to 2 weeks light protected and tightly capped at +2 to +8 C. If the calibrators cannot be used within this period, they should be aliquoted and stored frozen below -18 °C (up to 3 months). For more details about storage conditions please check your instruction manual.