3PLUS1® Multilevel Serum Calibrator Set

3PLUS1® Multilevel Serum Calibrator Set

Order no.: 62028/RUO
  • 25-OH-vitamin D2
  • 25-OH-vitamin D3

MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

Description

3PLUS1® Multilevel Serum Calibrator Set, 25-OH-Vitamin D3/D2, lyophilised

These lyophilised calibrators from Chromsystems are based on human serum and should be used according to the instruction manual. They are designed to calibrate the measurement method for the quantitative determination of 25-OH-vitamin D3 and 25-OH-vitamin D2. While following the instructions the calibrators should be handled and measured in the same manner as research samples.
These calibrators are only available as multilevel calibrator set with 3 different concentration levels plus 1 blank value.

Technical Data

Method of Analysis

LC-MS/MS

Content

4 x 1 ml (lyoph.)

Parameter

25-OH-vitamin D2, 25-OH-vitamin D3

Concentrations

Please note: Concentration values shown here are typical. For lot specific concentration values please refer to the product insert supplied with the materials. The product inserts can also be requested from us directly.

SubstanceUnitCalibrator 1Calibrator 2Calibrator 3Blank Calibrator
25-OH-Vitamin D3 µg/l
nmol/l
18.1
45.2
32.0
79.8
65.0
162
n.d.
25-OH-Vitamin D2 µg/l
nmol/l
14.4
34.9
28.4
68.8
56.0
136
n.d.

n.d. = not detected (< LOQ)

Reconstitution

Carefully remove the metal seal and rubber stopper from the vials. (Caution when removing the metal seal: risk of injuries due to sharp cutting edges!). Add exactly 1.0 ml of distilled water into each vial. Return the stopper and incubate for 10 to 15 minutes at room temperature. Swirl the vials to dissolve the contents until homogeneity.

Storage conditions

Stored below -18 °C and unopened, the lyophilised calibrators are stable until the expiry date specified on the vial labels. The reconstituted calibrators can be stored up to 1 week tightly capped at +2 to +8 C. If the calibrators cannot be used within this period, they should be aliquoted and stored frozen below -18 °C (up to 3 months). For more details about storage conditions please check your instruction manual.