92025XT LCMS TDM Series A 3PLUS1 antiepileptic drugs

3PLUS1® Multilevel Plasma Calibrator Set Antiepileptic Drugs/EXTENDED

Order no.: 92025/XT
  • 10,11-Dihydroxycarbamazepine
  • 10-OH-carbamazepine
  • Brivaracetam
  • Carbamazepine
  • Carbamazepine-10,11-epoxide
  • Ethosuximide
  • Felbamate
  • Gabapentin
  • Lacosamide
  • Lamotrigine
  • Levetiracetam (Keppra®)
  • N-Desmethylmesuximide
  • Oxcarbamazepine
  • Perampanel
  • Phenobarbital
  • Phenylethylmalonamide (PEMA)
  • Phenytoin
  • Pregabalin
  • Primidone
  • Retigabine
  • Rufinamide
  • Stiripentol
  • Sultiame
  • Theophylline
  • Tiagabine
  • Topiramate
  • Valproic acid
  • Vigabatrin
  • Zonisamide
 

Description

3PLUS1® Multilevel Plasma Calibrator Set, lyophilised

 

These lyophilised calibrators from Chromsystems are based on human plasma and should be used according to the instruction manual. They are designed to calibrate the measurement method in clinical diagnostics for the quantitative determination of antiepileptic drugs in serum/plasma. While following the instructions the calibrators should be handled and measured in the same manner as a patient specimen.
These calibrators are only available as multilevel calibrator set with 3 different concentration levels plus 1 blank value.

Technical Data

Method of Analysis

LC-MS/MS

Content

4 x 1 ml (lyoph.)

Parameter

10,11-Dihydroxycarbamazepine, 10-OH-carbamazepine, Brivaracetam, Carbamazepine, Carbamazepine-10,11-epoxide, Ethosuximide, Felbamate, Gabapentin, Lacosamide, Lamotrigine, Levetiracetam (Keppra®), N-Desmethylmesuximide, Oxcarbamazepine, Perampanel, Phenobarbital, Phenylethylmalonamide (PEMA), Phenytoin, Pregabalin, Primidone, Retigabine, Rufinamide, Stiripentol, Sultiame, Theophylline, Tiagabine, Topiramate, Valproic acid, Vigabatrin, Zonisamide

Concentrations

Please note: Concentration values shown here are typical. For lot specific concentration values please refer to the product insert supplied with the materials. The product inserts can also be requested from us directly or downloaded from our website after login.

Substance

Calibrator 1

(mg/l)

Calibrator 2

(mg/l)

Calibrator 3

(mg/l)

Blank Calibrator

(mg/l)

10-OH-Carbamazepine

3.43

25.1

45.7

n.d. (<0.500)

Carbamazepine

1.62

8.35

14.0

n.d. (<0.371)

Carbamazepine-10.11-epoxide

0.158

5.64

10.3

n.d. (<0.021)

Carbamazepine-Diol

0.137

5.60

12.2

n.d. (<0.052)

Oxcarbazepine

0.137

1.83

3.60

n.d. (<0.020)

Felbamate

12.9

68.1

121

n.d. (<1.100)

Lacosamide

0.735

6.25

11.2

n.d. (<0.092)

Lamotrigine

1.51

9.66

17.0

n.d. (<0.192)

Levetiracetam

5.06

30.5

100.2

n.d. (<0.750)

Rufinamide

3.99

20.5

34.1

n.d. (<0.506)

Theophylline

6.22

15.7

24.7

n.d. (<1.01)

Gabapentin

1.58

14.5

25.5

n.d. (<0.315)

Pregabalin

0.146

6.35

12.2

n.d. (<0.025)

Sultiame

0.371

8.10

15.0

n.d. (<0.075)

Tiagabine (in µg/l)

13.5

126

230

n.d. (<2.00)

Topiramate

0.642

15.6

29.4

n.d. (<0.191)

Vigabatrin

1.48

22.7

41.2

n.d. (<0.297)

N-Desmethylmesuximide

6.99

27.5

47.3

n.d. (<1.42)

PEMA

0.997

6.31

11.2

n.d. (<0.149)

Phenytoin

3.45

13.9

23.4

n.d. (<0.715)

Primidone

3.06

11.4

18.7

n.d. (<0.502)

Stiripentol

2.80

14.4

24.1

n.d. (<0.413)

Ethosuximide

20.3

66.4

110

n.d. (<6.11)

Phenobarbital

6.34

30.8

55.1

n.d. (<0.925)

Valproic acid

28.4

73.4

120

n.d. (<8.83)

Zonisamide

3.73

27.6

49.1

n.d. (<1.43)

Brivaracetam

0.618

2.42

4.19

n.d. (<0.074)

Perampanel (in µg/l)

35.7

1003

1780

n.d. (<20.0)

Retigabine (in µg/l)

48.7

1058

2050

n.d. (<28.0)

n.d. = not determined, analytes under LOQ

The blank level is manufactured using the same matrix pool as used for the calibrator levels 1, 2 and 3. It was not fortified with any antiepileptic drugs.

Reconstitution

Remove the rubber stopper from the vial. Add exactly 1.0 ml of distilled water into the vial. Replace the stopper and allow to sit for 10 to 15 minutes at room temperature. Swirl the vial to dissolve the contents until homogeneity.

Storage conditions

Stored below –18 °C unopened calibration standards are stable until the expiry date which is stated on the vial labels. The reconstituted calibration standards when kept light protected, well closed and cool (+2 to +8 °C) are stable for 2 days. If the calibrators cannot be analysed within this period, they should be aliquoted and stored frozen below -18 °C (up to 3 months).