65765 F HPLC kit vitamin C

Vitamin C in Plasma/Serum - Automated HPLC

Order no.: 65765/F
480 Tests
  • Vitamin C

Automated sample preparation with 96 well filter plates

Description

Clinical relevance

Vitamin C (ascorbic acid, ascorbate) is a water-soluble vitamin that is an essential cofactor for the human body, for instance in the catabolism of tyrosine and the synthesis of catecholamines. Regular consumption of fruit and vegetables is usually sufficient to cover the body’s vitamin C needs.

In the case of vitamin C deficiency, procollagen chains are produced insufficiently and stable fibrils cannot be formed in the affected cells. Without functioning collagen, the blood vessels and tendons become fragile and the typical skin-related symptoms associated with scurvy start to develop. In addition to the collagen damages described above, further diseases caused by vitamin C deficiency include bleeding of uncertain origin, loss of teeth and overactive thyroid gland (hyperthyroidism). Vitamin C deficiency is also observed in dialysis patients, in chronic alcohol abuse and during pregnancy.

 

Product advantages

  • High stability of processed samples and controls
  • Enhanced column stability
  • Reliable quantification with a stable internal standard

 

This Chromsystems kit allows the reliable chromatographic determination of vitamin C in one isocratic HPLC run with UV detection. An efficient protein precipitation step removes interfering components and stabilises Vitamin C simultaneously. A stable internal standard is used for reliable quantification. Due to a special stabilisation of the prepared samples, it is also possible to reliably process and measure large sample batches.

 

Kit content

Mobile Phase, 1000 ml
Vitamin C Plasma Calibration Standard (lyoph.), 5 x 0.5 ml
Internal Standard, 10 ml
96 Well filter plates, 5 pcs.
Collection plates, 5 pcs.
Pierceable adhesive seals for 96 well plates, 5 pcs.

Chromatogram

Technical Data

Method of Analysis

HPLC

Number of Tests

480

Parameter

Vitamin C

Analysis Time

5 min

Limit of quantification

0.5 mg/l

Linearity

up to 120 mg/l

Intraassay

CV <= 3 %

Interassay

CV < 6 %

Gradient

Isocratic

Information

Any isocratic HPLC system with UV detection is suitable.

Column temperature

ambient (~25 °C)

Flow rate

1.5 ml/min

Specimen

Plasma/Serum

Pre-analytic Treatment

Specimens: Fresh lithium heparin plasma

Sample stability: 5 days at -20 °C, up to 2 hours at room temperature
Detailed information on stability and transportation can be obtained from the instruction manual.

Sample Preparation

  • Place 100 µl Internal Standard in a 96 well filter plate.
  • Add 100 µl sample/calibrator/control.
  • Vortex for 30 s at 1100 rpm (orbit: 2 mm).
  • Centrifuge for 5 min at 2000 x g.
  • Close the 96 well filter plate.
  • Vortex for 30 s at 800 rpm (orbit: 2 mm).
  • Inject 20 µl of the prepared sample into the HPLC system.

Injection Volume

20 µl

Flow

1.5 ml/min

Additional Info

For the Chromsystems HPLC analysis of vitamin C any isocratic HPLC system with UV detection is suitable.

Wavelength

245 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Calibrators

Calibrators available separately

Controls

Controls available separately

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