34000-premix HPLC kit vitamin A vitamin E serum plasma

One Step Vitamins A and E in Serum/Plasma - HPLC

Order no.: 34000/Premix
100 Tests
  • Vitamin A
  • Vitamin E

With ready-to-use pre-mixed tubes
Only one pipetting step
Fully automated workflow available

Description

Clinical relevance

Vitamins A and E are fat-soluble vitamins, which perform important functions in the body.

Vitamin A plays an important role in vision, bone metabolism, and the synthesis of steroid hormones. Deficiency of vitamin A can lead to night blindness, skin dehydration, and hair loss.

Vitamin E protects, among others, unsaturated fatty acids in the cell membranes and the LDL cholesterol from attacks by reactive radicals, which can occur as a result of increased oxidative stress in the body. Deficiency of vitamin E can lead to permanent joint pain, making the determination of vitamin E levels essential in this case.

However, overdoses of these vitamins may cause hypervitaminosis with intoxication symptoms. In addition, chemotherapies should not be "accompanied" by extra vitamin supplements – especially high doses of vitamins A and E may limit the success of treatment.

 

Product advantages

  • Extremely simple sample preparation in a single step
  • Easy handling
  • Low analysis costs
  • Automated version available

 

This Chromsystems kit allows the simultaneous analysis of vitamins A and E in serum/plasma in a single isocratic HPLC run with UV detection. The special kit feature: Sample preparation is performed very fast and simply in one single pipetting step using premixed tubes. Additionally, a stable internal standard is used for precise quantification.

Chromsystems offers additional kits for the fast and efficient determination of vitamins A and E. You can choose between several options depending on the device capabilities (HPLC or UHPLC) and the sample quantities in your laboratory. Alternatively, the standard kit 34000 is available, as well as the 34900/UHPLC kit for higher sample throughput using UHPLC determination. Our kits are also available with automated workflow (34700/F and 34900/F).

Chromatogram

Technical Data

Method of Analysis

HPLC

Number of Tests

100

Parameter

Vitamin A, Vitamin E

Analysis Time

9 min

Limit of quantification

Vitamin A: 0.02 mg/l
Vitamin E: 0.60 mg/l

Linearity

Vitamin A: up to 4.5 mg/l
Vitamin E: up to 38 mg/l

Recovery

Vitamin A > 99 %; Vitamin E > 96 %

Intraassay

CV < 6 %

Interassay

CV < 5 %

Column temperature

ambient (~ 25 °C)

Flow rate

1.50 ml/min

Specimen

Serum/Plasma

Pre-analytic Treatment

Time of sample drawing

  • In the morning before breakfast
  • Prior to any medication

Collection and storage of specimens

  • Collect about 1 to 2 ml blood.
  • Centrifugate 5 min at 2000 rpm.
  • Store serum/plasma below -18 °C and protect from light until analysed.

Sample Preparation

  • Pipette 100 µl serum/plasma into a Pre-mixed Reaction Tube.
  • Vortex 30 s.
  • Centrifuge 10 min at at least 9000 x g.
  • Transfer supernatant into a light protected autosampler vial and inject 50 µl into the HPLC system.

Injection Volume

50 µl

Additional Info

For the analysis of vitamins A and E any isocratic HPLC system with UV detector is suitable. As these vitamins are detected at different wavelengths, a detector with programmable wavelength switching is very useful for simultaneous analysis of vitamins A and E in a single HPLC run.

Wavelength

325 nm, after 3.5 min switch to 295 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Calibrators

Calibrators available separately

Controls

Controls available separately

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