64000 MassChrom® Methylmalonic Acid in Serum/Plasma and Urine - LC-MS/MS

MassChrom® Methylmalonic Acid in Serum/Plasma and Urine - LC-MS/MS

Order no.: 64000
400 Tests
  • Methylmalonic acid (MMA)

Clean-up tubes for fast preparation of plasma and serum samples
Direct injection of urine samples after dilution
Chromatographic separation of succinic acid

Description

Clinical relevance

Methylmalonic acid (MMA) is an early, sensitive biomarker of vitamin B12 deficiency. Vitamin B12 deficiency leads to high concentrations of MMA in plasma, serum and urine. MMA is much more suitable for diagnosing a subclinical vitamin deficiency than the measurement of vitamin B12 itself, which is bound to various  proteins in the blood and is significantly more unstable. Furthermore, MMA is a more sensitive biomarker, which indicates vitamin B12 deficiency earlier.

Vitamin B12 deficiency that remains untreated for years can lead to many clinical symptoms. Neurological and physical disorders have been observed, as well as anaemia and pregnancy complications up to miscarriages. Together with vitamin B6 and folic acid, vitamin B12 also plays a role in Alzheimer’s disease. Therefore, early determination of the MMA level and early treatment can prevent certain diseases and irreversible neurological damages.

 

Product advantages

  • Clean-Up Tubes for fast processing of plasma and serum samples
  • Direct injection of urine samples after dilution
  • Chromatographic separation of succinic acid
  • 3PLUS1® Multilevel Calibrator Sets for plasma and urine

 

This Chromsystems kit allows the specific determination of methylmalonic acid in plasma, serum and urine. The use of special clean-up tubes enables removing interfering compounds fast and effectively. The isobaric succinic acid is separated chromatographically to prevent false-positive test results. A stable isotopically labelled internal standard is used to ensure reliable quantification. Matrix effects are minimised and the precision and robustness of the method are optimised. The human-based 3PLUS1® multilevel plasma and urine calibrators ensure high precision of the results.

Please see accessories for the additional urine upgrade set.

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Number of Tests

400

Parameter

Methylmalonic acid (MMA)

Analysis Time

3.5 min

Limit of quantification

Plasma/Serum: 3.5 µg/l
Urine: 86 µg/l

Linearity

Plasma/Serum: up to 600 µg/l
Urine: up to 9500 µg/l

Recovery

Plasma/Serum: 104 %; Urine: 98 %

Intraassay

Plasma/Serum: CV = 2.3-2.5 %
Urine: 1.8-2.2 %

Interassay

Plasma/Serum: CV = 3.2-3.6 %
Urine: CV = 2.4-2.9 %

Gradient

0 min 100 % Mobile Phase A
2.5 min 0 % Mobile Phase A, 100 % Mobile Phase B
2.51-3.5 min 100 % Mobile Phase A

Column temperature

ambient temperature (approx. 25 °C)

Flow rate

0.7 ml/min

Specimen

Plasma/Serum/Urine

Sample Preparation

Plasma/serum:

  • Pipette 100 µl sample/calibrator/control into Clean-Up Tubes
  • Add 50 µl Internal Standard and mix (vortex) briefly
  • Centrifuge 10 min at 14 000 x g
  • Transfer filtrate into an autosampler vial

Urine:

  • Pipette 200 µl sample/calibrator/control into transparent reaction vials
  • Add 25 µl Internal Standard and mix 10 s (vortex)
  • Dilute sample with Dilution Buffer in an autosampler vial

Sample Stability

At +2 to +8 °C up to 14 days, below -18 °C up to 2 months.

Injection Volume

10 to 20 µl

Ionisation

ESI negative

MS/MS-Mode

MRM

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Calibrators

Controls

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