32000 HPLC kit ß-carotene serum plasma

β-Carotene in Serum/Plasma - HPLC

Order no.: 32000
100 Tests
  • Beta-carotene

Fast and easy sample preparation
Low costs per test
High sample stability
Further analytes detectable: Cryptoxanthin, Lycopene, α-Carotene, cis-β-Carotene

Description

Clinical relevance

β-carotene (provitamin A) is a precursor form of vitamin A, and is an essential nutrient for the human diet. Like glutathione or vitamin C, it possesses antioxidative properties. β-carotene is a herbal dye used as a colouring in the food industry and in vitamin products.

β-carotene can only be processed by the body when absorbed together with fat. Any additional high-dosed preventive therapy should be used with caution to prevent a possible overdose. Additional use of high dose β-carotene over several years combined with nicotine and alcohol consumption may even increase the risk of lung or bowel cancer. For this reason, medications that contain β-carotene are labelled with a warning for smokers. Furthermore, drugs with high concentrations of β-carotene are no longer allowed to be prescribed to smokers either.

 

Product advantages

  • Simple, fast and effective sample preparation
  • High sample stability
  • Low costs per test

 

This reagent kit is designed for easy and reliable determination of β-carotene in serum/plasma. The sample clean up procedure combines a fast precipitation and extraction step. The resulting extract is soluble in the mobile phase, so no evaporation is necessary. The chromatographic determination is run on an isocratic HPLC system with UV/VIS detection. A special RP-chromatography ensures a reliable separation of α-, cis-β-, and all-trans-β-carotene as well as some further carotenoids in less than 10 minutes. The analytes are quantified by the inclusion of an internal standard, which is a non-naturally occurring carotenoid-derivative, so that only a single detection wavelength is required. The high stability of the processed samples allows for easier performance of large sample batches.

 

Kit content

Mobile Phase, 1000 ml
β-Carotene Serum Calibration Standard (lyoph.), 5 x 0.5 ml
Internal Standard, 5 ml
Precipitation Reagent, 5 ml
Extraction Buffer, 20 ml
Reaction vials, amber coloured (light protection), 100 pcs.

Chromatogram

Technical Data

Method of Analysis

HPLC

Number of Tests

100

Parameter

Beta-carotene

Analysis Time

approx. 10 min

Limit of quantification

10 ng/ml

Linearity

up to 3000 ng/ml

Recovery

> 90 %

Intraassay

CV = 4.8 %

Interassay

CV < 4.2 %

Information

Stability of the prepared samples: 2 days at ambient temperature (light protected)

Column temperature

ambient (~25 °C)

Flow rate

1.5-1.8 ml/min

Specimen

Serum/Plasma

Sample Preparation

Sample preparation

  • Pipette 100 µl serum/plasma into a light protected reaction vial.
  • Add 50 µl internal standard, mix briefly.
  • Add 50 µl Precipitation Reagent, vortex briefly (do not centrifuge!).
  • Add 200 µl Extraction Buffer, vortex well about 30 s.
  • Centrifuge 10 min at 13000 rpm.
  • Transfer the supernatant into a light protected autosampler vial (glass).
  • Inject 50 µl into the HPLC system.

Sample Stability

At +2 to +8 °C up to 5 days. Keep specimens frozen and light protected for transport and freeze for longer storage.

Injection Volume

50 µl

Additional Info

For the HPLC analysis of ß-carotene/carotenoids any isocratic HPLC system with UV/VIS detector is suitable.

Wavelength

453 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Mobile Phase


Order no.: 32001

Internal Standard


Order no.: 32004

Precipitation Reagent


Order no.: 32005

Extraction Buffer


Order no.: 32006

Reaction vials


Order no.: 33005

Calibrators

Calibrators available separately

Controls

Controls available separately

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