Parameter Set Anti-HIV Drugs - LC-MS/MS

Parameter Set Anti-HIV Drugs - LC-MS/MS

   
  • Amprenavir
  • Atazanavir
  • Darunavir
  • Delavirdine
  • Efavirenz
  • Elvitegravir
  • Etravirine
  • Indinavir
  • Lopinavir
  • Maraviroc
  • Nelfinavir
  • Nelfinavir-M8
  • Nevirapine
  • Raltegravir
  • Rilpivirine
  • Ritonavir
  • Saquinavir
  • Tipranavir

Order no.: 92924, 92111, 92110

Encompasses 18 analytes
6PLUS1® Multilevel Calibrator Set available
Part of the MassTox® TDM Series A

Product Name Qty
Parameter Set - Anti-HIV Drugs - LC-MS/MS
Order no.: 92924
BASIC Kit A for 200 tests - LC-MS/MS
Order no.: 92111/200
BASIC Kit A for 1000 tests - LC-MS/MS
Order no.: 92111/1000
MassTox® TDM MasterColumn® A
Order no.: 92110

Description

Clinical relevance

According to estimates from UNAIDS there are about 36.9 million people worldwide living with HIV, of which approximately 3 million are children under the age of 15 years. Even if the number of globally registered new infections is decreasing, it was still at approx. 2 million in 2014. The currently available drugs, which are usually prescribed as part of HAART (highly active antiretroviral therapy), interfere with or prevent the development of the virus at different stages of reproduction or cell infection.

Regular monitoring of drug concentration is very important, especially in HIV therapy. Sufficiently high levels of the administered antiretroviral drugs in plasma are a key factor in the success of the treatment. Individual plasma levels can fluctuate significantly and low levels can affect the success of the treatment. On the other hand high concentrations can cause major side effects, such as disorders of the central nervous system, liver toxicity, gastrointestinal disorders or renal toxicity. Therefore the measurement of drug concentrations in serum or plasma is essential for optimising the therapy.

 

Product advantages

With the Parameter Set for the LC-MS/MS analysis of anti-HIV drugs in serum/plasma, 18 different drugs can be measured quickly and efficiently. Due to the careful optimisation of all kit components as well as the chromatographic separation, matrix effects (“ion suppression”) are minimised and the robustness of the method is enhanced. Sample preparation is based on a protein precipitation step. The use of stable isotopically labelled (deuterated) and co-eluting internal standards as well as 6PLUS1® multilevel calibrators ensures high precision and the reproducible and reliable quantification of the analytes. The method is comprehensively validated.

The Parameter Set is a part of the Series A modular system, which enables the analysis of more than 150 parameters without switching column or changing the mobile phases, thereby minimising required workload in the laboratory. The Basic Kit A contains all components required for sample preparation and all necessary mobile phases. The MasterColumn® A is the analytical column used for the determination of all Series A analytes. Our portfolio contains further MassTox® Parameter Sets for covering more than 150 substances and metabolites.

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Parameter

Amprenavir, Atazanavir, Darunavir, Delavirdine, Efavirenz, Elvitegravir, Etravirine, Indinavir, Lopinavir, Maraviroc, Nelfinavir, Nelfinavir-M8, Nevirapine, Raltegravir, Rilpivirine, Ritonavir, Saquinavir, Tipranavir

Analysis Time

3.0–3.5 min

Linearity

at least twice the therapeutic range

Intraassay

CV= 1.0–6.7 %

Interassay

CV= 1.5–8.1 %

Gradient

Group 1
0.00–0.20 min, 0 % Mobile Phase 2
0.21–2.00 min, 70 % Mobile Phase 2
2.01–2.90 min, 100 % Mobile Phase 2
2.91–3.50 min, 0 % Mobile Phase 2
Group 2
0.00–0.20 min, 0 % Mobile Phase 2
0.21–0.60 min, 50 % Mobile Phase 2
0.61–2.40 min, 90 % Mobile Phase 2
2.41–3.00 min, 0 % Mobile Phase 2
Group 3
0.00–0.20 min, 0 % Mobile Phase 2
0.21–2.30 min, 90 % Mobile Phase 2
2.31–3.10 min, 0 % Mobile Phase 2

Specimen

Serum/Plasma

Sample Preparation

  • Prepare the Internal Standard Mix
  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 μl sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 μl Extraction Buffer
  • Mix and incubate 2 min at ambient temperature (do not centrifuge)
  • Add 250 μl of mixture A and mix 30 s minimum (vortex)
  • Centrifuge 5 min at 15000 x g.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity

Sample Stability

Samples are stable up to 7 days at +2 to +8 °C. For longer storage periods keep samples frozen below -18 °C.

Injection Volume

15 - 25 μl

Ionisation

ESI positive

MS/MS-Mode

MRM

Additional Info

We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Calibrators

Calibrators available separately

Controls

Controls available separately

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