Parameter Set Tricyclic Antidepressants TCA 2 - LC-MS/MS

Parameter Set Tricyclic Antidepressants TCA 2 - LC-MS/MS

   
  • Clomipramine
  • Maprotiline
  • Norclomipramine
  • Normaprotiline
  • Nortrimipramine
  • Protriptyline
  • Trimipramine

Order no.: 92920, 92111, 92110

Encompasses 7 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the MassTox® TDM Series A

Product Name Qty
Parameter Set - Tricyclic Antidepressants TCA 2 - LC-MS/MS
Order no.: 92920
BASIC Kit A for 200 tests - LC-MS/MS
Order no.: 92111/200
BASIC Kit A for 1000 tests - LC-MS/MS
Order no.: 92111/1000
MassTox® TDM MasterColumn® A
Order no.: 92110

Description

Clinical relevance

Tricyclic antidepressants (TCAs) are used to treat psychological conditions such as anxiety, pain syndromes, and phobias, and produce a positive mood enhancing effect. Their pharmacological effect is based on increasing the concentration of dopamine, noradrenaline and serotonin in the synaptic cleft by inhibiting the degradation or reuptake of these neurotransmitters. TCAs are highly lipophilic and are absorbed rapidly from the gastrointestinal tract after oral administration. Even before they reach their site of action in the brain, a fraction is already metabolised in the liver (first-pass effect). Regular determination of the plasma levels can minimise the risk of side effects resulting from overdose. Furthermore, patient compliance can be checked by this procedure to help ensure optimal therapeutic success.

 

Product advantages

With the Parameter Set Tricyclic Antidepressants TCA 2 for the LC-MS/MS method in serum/plasma, 7 different drugs can be measured quickly and efficiently. Due to the careful optimisation of all kit components as well as the chromatographic separation, matrix effects (“ion suppression”) are minimised and the robustness of the method is enhanced. Sample preparation is based on a protein precipitation step. The use of stable isotopically labelled (deuterated) and co-eluting internal standards as well as 3PLUS1® multilevel calibrators ensures high precision and the reproducible and reliable quantification of the analytes. The method is comprehensively validated.

The Parameter Set is a part of the Series A modular system, which enables the analysis of more than 150 parameters without switching column or changing the mobile phases, thereby minimising required workload in the laboratory. The Basic Kit A contains all components required for sample preparation and all necessary mobile phases. The MasterColumn® A is the analytical column used for the determination of all Series A analytes. Our portfolio contains further MassTox® Parameter Sets for covering more than 150 substances and metabolites.

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Parameter

Clomipramine, Maprotiline, Norclomipramine, Normaprotiline, Nortrimipramine, Protriptyline, Trimipramine

Analysis Time

3.5 min

Limit of quantification

1.5 - 10 µg/l

Linearity

at least twice the therapeutic range

Intraassay

CV = 1.7 - 4.3 %

Interassay

CV = 3.8 - 6.3 %

Gradient

Isocratic (35 % Mobile Phase 1; 65 % Mobile Phase 2)

Specimen

Serum/Plasma

Sample Preparation

  • Prepare the Internal Standard Mix
  • Add 800 µl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 µl sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 µl Extraction Buffer
  • Mix and incubate 2 min at ambient temperature (do not centrifuge)
  • Add 250 µl of mixture A and mix 30 s minimum (vortex)
  • Centrifuge 5 min at 9000 x g
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity

Sample Stability

Samples are stable up to 1 week at +2 to +8 °C. For longer storage periods keep samples frozen below -18 °C.

Injection Volume

5 - 100 μl

Ionisation

ESI positive

MS/MS-Mode

MRM

Additional Info

We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Calibrators

Controls

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