92918 LCMS TDM Series A Benzodiazepines2

Parameter Set Benzodiazepines 2 - LC-MS/MS

   
  • Alprazolam
  • Bromazepam
  • Clonazepam
  • Desalkylflurazepam
  • Flunitrazepam
  • Flurazepam
  • Lorazepam
  • Lormetazepam
  • Nitrazepam
  • Triazolam

Order no.: 92918, 92111, 92110

Encompasses 10 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the MassTox® TDM Series A

Product Name Qty
Parameter Set - Benzodiazepines 2 - LC-MS/MS
Order no.: 92918
BASIC Kit A for 200 tests - LC-MS/MS
Order no.: 92111/200
BASIC Kit A for 1000 tests - LC-MS/MS
Order no.: 92111/1000
MassTox® TDM MasterColumn® A
Order no.: 92110

Description

Clinical relevance

Benzodiazepines (BZD) are a group of psychoactive drugs with similar structures that are classified as sedatives. By virtue of their high lipophilicity they penetrate the blood-brain barrier easily and, after being resorbed, quickly reach the central nervous system, where they enhance the impact of the inhibiting neurotransmitter GABA. Besides anxiolytic and sedative effects, BZD also have anti-aggressive, hypnotic, muscle relaxant and anticonvulsive properties. The predominance of the individual effects varies with the special structures of the benzodiazepines and the administered dose. BZD in blood are present mostly bound nonspecifically to plasma proteins. They are metabolised mainly by the liver. The resulting metabolites are mostly pharmacologically active themselves, and in some cases they represent the actual active substance, e.g. nordiazem being metabolised from diazepam or clorazepate. The plasma elimination times are highly variable: from a few hours (midazolam) to several days (clobazam). The metabolites frequently have longer half-lives than the parent substance, so an enrichment of therapeutically effective substances in the blood can occur.

 

Product advantages

With the Parameter Set Benzodiazepines 2 for the LC-MS/MS method in serum/plasma, 10 different drugs can be measured quickly and efficiently. By carefully optimising all of the kit components and the chromatographic separation, matrix effects (“ion suppression”) have been minimised and the robustness of the method has been enhanced. Sample preparation is based on protein precipitation. The use of stable isotopically labelled (deuterated) and co-eluting internal standards as well as 3PLUS1® multilevel calibrators ensures high precision and reproducible and reliable quantification of the analytes. The method is comprehensively validated.

The Parameter Set is a part of the Series A modular system, which enables the analysis of more than 170 parameters without switching column or changing the mobile phases, thereby minimising required workload in the laboratory. The Basic Kit A contains all components required for sample preparation and all necessary mobile phases. The MasterColumn® A is the analytical column used for the determination of all Series A analytes. Our portfolio contains further MassTox® Parameter Sets for covering more than 170 substances and metabolites.

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Parameter

Alprazolam, Bromazepam, Clonazepam, Desalkylflurazepam, Flunitrazepam, Flurazepam, Lorazepam, Lormetazepam, Nitrazepam, Triazolam

Analysis Time

2.8 min

Limit of quantification

1–10 µg/l

Linearity

up to 50–750 μg/l

Intraassay

CV = 2–9 %

Interassay

CV = 6–14 %

Gradient

0.00 - 0.30 min     20 % Mobile Phase 2
0.31 - 0.60 min     50 % Mobile Phase 2
0.61 - 1.10 min     80 % Mobile Phase 2
1.11 - 2.00 min   100 % Mobile Phase 2
2.01 - 2.21 min,  100 % -> 20 % Mobile Phase 2
2.21 - 2.80 min     20 % Mobile Phase 2

Specimen

Serum/Plasma

Pre-analytic Treatment

Stored in the dark and cooled (+4°C) patient samples are stable for at least 24 hours. For longer storage periods keep patient samples frozen below -18°C.

Sample Preparation

  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 μl sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 μl Extraction Buffer, mix briefly (vortex) and incubate 2 min
  • Add 250 μl of mixture A, mix 30 s (vortex) and centrifuge 5 min
  • Dilute the supernatant with Dilution Buffer (depending on the instrument sensitivity) and inject into LC-MS/MS system.

Sample Stability

Samples are stable up to 1 week at +2 to +8 °C. For longer storage periods keep samples frozen below -18 °C.

Injection Volume

0.2–50 μl

Ionisation

ESI positive

MS/MS-Mode

MRM

Additional Info

We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Calibrators

Calibrators available separately

Controls

Controls available separately