Parameter Set Antidepressants 2/Psychostimulants - LC-MS/MS

Parameter Set Antidepressants 2/Psychostimulants - LC-MS/MS

   
  • Atomoxetine
  • Methylphenidate
  • Mianserin
  • Reboxetine
  • Ritalinic acid
  • Trazodone

Order no.: 92915, 92111, 92110

Encompasses 6 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the MassTox® TDM Series A

Product Name Qty
Parameter Set - Antidepressants 2/Psychostimulants - LC-MS/MS
Order no.: 92915
BASIC Kit A for 200 tests - LC-MS/MS
Order no.: 92111/200
BASIC Kit A for 1000 tests - LC-MS/MS
Order no.: 92111/1000
MassTox® TDM MasterColumn® A
Order no.: 92110

Description

Clinical relevance

In addition to several antidepressants, this parameter set also includes psychostimulants such as methylphenidate (Ritalin®). The amphetamine-like substance is mainly used in the treatment of attention deficit/hyperactivity disorder (ADHD). Methylphenidate inhibits the reuptake of dopamine and noradrenaline, thus increasing their concentration in the synaptic cleft and enhancing the activity of the sympathetic nervous system. Atomoxetine, which has a chemical structure similar to fluoxetine, is another drug used to treat ADHD. In Germany, atomoxetine is approved for ADHD and is not subject to the controlled-substances law of Germany (Betäubungsmittelgesetz BtMG). Mianserin, reboxetine, and trazodone are prescribed for depression.

 

Product advantages

With the Parameter Set Antidepressants 2/Psychostimulants in serum/plasma, 6 different drugs can be measured quickly and efficiently using LC-MS/MS. Due to the careful optimisation of all kit components as well as the chromatographic separation, matrix effects (“ion suppression”) are minimised and the robustness of the method is enhanced. Sample preparation is based on a protein precipitation step. The use of stable isotopically labelled (deuterated) and co-eluting internal standards as well as 3PLUS1® multilevel calibrators ensures high precision and the reproducible and reliable quantification of the analytes. The method is comprehensively validated.

The Parameter Set is a part of the Series A modular system, which enables the analysis of more than 150 parameters without switching column or changing the mobile phases, thereby minimising required workload in the laboratory. The Basic Kit A contains all components required for sample preparation and all necessary mobile phases. The MasterColumn® A is the analytical column used for the determination of all Series A analytes. Our portfolio contains further MassTox® Parameter Sets for covering more than 150 substances and metabolites.

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Parameter

Atomoxetine, Methylphenidate, Mianserin, Reboxetine, Ritalinic acid, Trazodone

Analysis Time

2.8 min

Limit of quantification

1-4 μg/l

Linearity

250-5000 μg/l

Intraassay

CV 2.9-6.2 %

Interassay

CV 3.3-6.9 %

Gradient

0.00 min                  30 % Mobile Phase 2
0.00 →1.00 min   100 % Mobile Phase 2
1.01-2.10 min       100 % Mobile Phase 2
2.11-2.80 min         30 % Mobile Phase 2

Specimen

Serum/Plasma

Pre-analytic Treatment

Stored in the dark and cooled at +4 °C patient specimens are stable for at least 24 hours. For longer storage periods the patient specimens should be frozen at ca. -20 °C. It is mandatory to ship and keep patient specimens frozen when they have to be measured for methylphenidate and ritalinic acid.

Sample Preparation

  • Prepare the Internal Standard Mix

  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A

  • Pipette 50 μl sample/calibrator/MassCheck® control into a 1.5 ml reaction vial

  • Add 25 μl Extraction Buffer

  • Mix and incubate 2 min at ambient temperature (do not centrifuge)

  • Add 250 μl of mixture A and mix 30 s minimum (vortex)

  • Centrifuge 5 min at 9000 x g.

  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity

Sample Stability

Prepared samples are stable up to 3 days at ambient temperature and up to 5 days at +2 to +8 °C. For longer storage periods keep them frozen below -18 °C.

Injection Volume

0.2-50 μl

Ionisation

ESI positive

MS/MS-Mode

MRM

Additional Info

We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Calibrators

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