93900 MassTox® Immunosuppressants ONEMinute Test for automated sample preparation - LC-MS/MS

MassTox® Immunosuppressants ONEMinute Test for automated sample preparation - LC-MS/MS

Order no.: 93900/1200/DWP
1200 Tests
  • Cyclosporin A
  • Everolimus
  • Sirolimus
  • Tacrolimus

96 samples in approx. 45 minutes (liquid handling system dependent)
Suitable for many liquid handling devices
Separation by centrifugation
Fully validated automated workflow

Description

Clinical relevance

Immunosuppressants suppress the activity of the immune system in order to prevent organ transplant rejection. They are also used as therapeutic drugs for the treatment of autoimmune diseases. Therapeutic drug monitoring of immunosuppressants is indispensable for therapy following after organ transplantation. Besides mycophenolic acid, the immunosuppressants most commonly used following an organ transplantion include cyclosporin A (INN: Ciclosporin), tacrolimus (FK506), sirolimus and everolimus. Cyclosporin A and tacrolimus block the cellular signal transduction, which is required for the immune response, by inhibiting the enzyme calcineurin. For instance, it controls the synthesis of interleukin in the T cells during an immune response. In contrast, sirolimus, and its derivative everolimus, are calcineurin-independent and inhibit mTOR (mammalian target of rapamycin), an important enzyme of the intracellular signal transduction during the immune response.

The quantitative determination of these immunosuppressants should be performed only with LC-MS/MS, because the immunological tests vary considerably in terms of selectivity and specificity, and sometimes also detect inactive metabolites. The compensation of matrix effects also plays a key role in the diagnostic analysis of immunosuppressants from a complex matrix such as whole blood. It is therefore essential that interferences are reduced significantly to achieve precise and reliable quantification.

 

Product advantages

  • Up to 800 samples per day
  • Reduced laboratory work required, higher throughput
  • Reliable elimination of matrix effects
  • High sensitivity and specificity
  • Very short analysis time of 1-2 minutes

 

The ONEMINUTE MassTox® kit for the analysis of the four immunosuppressants leads to robust, precise and reproducible results with a run time of only 1-2 minutes per sample. The ONEMINUTE method saves time, especially for laboratories with high sample throughput. Manual sample preparation is reduced to a simple and effective protein precipitation step. The concentration of the analytes and the elimination of other interfering substances is performed ONLINE using a trap column, which massively minimises the matrix effects. The isotopically labelled internal standards compensate entirely for remaining ion suppression. The trap column is connected by a simple switching system to the HPLC column and the MS/MS system. The additional use of 6PLUS1® multilevel calibrators and the MassCheck® controls ensures highly precise results.

This test is also available with manual sample preparation (order no. 93900) and as a standard method (order no. 93000).

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Number of Tests

1200

Parameter

Cyclosporin A, Everolimus, Sirolimus, Tacrolimus

Sample Preparation

  • Transfer 50 μl sample/calibrator/MassCheck® control into the Deep Well Extraction Plate.
  • Add 25 μl Internal Standard Mix
    + 100 μl Extraction Buffer
    + 250 μl Precipitation Reagent.
  • Agitate the extraction plate for 3 min.
  • Separate precipitate by centrifugation for 3 min at 2000 x g.
  • Transfer supernatant into the Collection Plate, seal it and transfer to autosampler for injection into the LC-MS/MS system.

Injection Volume

5–50 µl

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Calibrators

Calibrators available separately

Controls

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