92912XT LCMS TDM Series A Neuroleptics 1_XT

Parameter Set Neuroleptics 1/EXTENDED - LC-MS/MS

Order no.: 92912/XT
  • 9-OH-risperidone
  • Aripiprazole
  • Clozapine
  • Dehydroaripiprazole
  • Haloperidol
  • N-Desmethylclozapine
  • N-Desmethylolanzapine
  • Norquetiapine
  • Olanzapine
  • Quetiapine
  • Risperidone

Encompasses 11 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the MassTox® TDM Series A


Clinical relevance

Neuroleptic agents, also known as antipsychotics, are used to treat psychosis and other psychological disorders such as severe manic depressive conditions accompanied by delusions and hallucinations. Their effect is based on influencing the synaptic conduction in the central nervous system. The neuroleptics bind to specific receptors of the neurotransmitters such as dopamine, preventing transmission of stimulus information and leading to inhibition of the transmission of neural signals.

To avoid interferences and to enable an accurate analysis, the neuroleptics are divided into two Parameter Sets, Neuroleptics 1/EXTENDED and Neuroleptics 2/EXTENDED 2. The present analysis set Neuroleptics 1/EXTENDED has been extended by the metabolites norquetiapine and dehydroaripiprazole.


Product advantages

With the Parameter Set Neuroleptics 1/EXTENDED in serum/plasma, 11 different analytes can be measured fast and efficiently using LC-MS/MS. Due to the careful optimisation of all kit components as well as the chromatographic separation, matrix effects (“ion suppression”) are minimised and the robustness of the method is enhanced. Sample preparation is based on a protein precipitation step. The use of stable isotopically labelled (deuterated) and co-eluting internal standards as well as 3PLUS1® multilevel calibrators ensures high precision and reproducible and reliable quantification of the analytes. The method is comprehensively validated.

The Parameter Set is a part of the Series A modular system, which enables the analysis of all parameters without switching column or changing the mobile phases, thereby minimising required workload in the laboratory. The Basic Kit A contains all components required for sample preparation and all necessary mobile phases. The MasterColumn® A is the analytical column used for the determination of all Series A analytes. Our portfolio contains further MassTox® Parameter Sets.

For the analysis you require the MassTox® TDM Basic Kit A, the specifc MassTox® TDM Parameter Set and the analytical column MassTox® TDM MasterColumn® A.


Technical Data

Method of Analysis



9-OH-risperidone, Aripiprazole, Clozapine, Dehydroaripiprazole, Haloperidol, N-Desmethylclozapine, N-Desmethylolanzapine, Norquetiapine, Olanzapine, Quetiapine, Risperidone

Analysis Time

3 min

Limit of quantification

0.5 – 35 μg/l


at least twice the therapeutic range


CV < 7 %


CV < 8 %



Sample Preparation

  • Reconstitute the Internal Standard Mix.
  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 μl sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 μl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 μl of mixture A and mix 30 s minimum (vortex) and centrifuge 5 min.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity

Sample Stability

Samples are stable for at least 24 hours when stored in the dark at +4 °C. For longer storage periods keep samples frozen below -18°C.

Injection Volume

0.2 – 50 µl


0.00 - 0.20 min    20 % Mobile Phase 2
0.21 - 0.70 min    50 % Mobile Phase 2
0.71 - 2.30 min    70 % Mobile Phase 2
2.31 - 3.00 min    20 % Mobile Phase 2


ESI positive



Additional Info

We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components


Calibrators available separately