Parameter Set - Antidepressants 2/ Psychostimulants/EXTENDED - LC-MS/MS

Parameter Set - Antidepressants 2/ Psychostimulants/EXTENDED - LC-MS/MS

Order no.: 92915/XT
  • Atomoxetine
  • Bupropion
  • Clomethiazole
  • Dosulepin
  • erythro-Dihydrobupropion
  • Hydroxybupropion
  • Methylphenidate
  • Mianserin
  • Milnacipran
  • Moclobemide
  • N-Desmethyldosulepin
  • Opipramol
  • Reboxetine
  • Ritalinic acid
  • threo-Dihydrobupropion
  • Tianeptine
  • Tranylcypromine
  • Trazodone
  • Vilazodone
  • Vortioxetine

Expanded parameter menu of 20 analytes
Additional internal standards for enhanced robustness
Part of the continuously updated MassTox® TDM Series A

Description

Clinical relevance

Antidepressants are a group of chemically different drugs used to treat depression, obsessive-compulsive disorder, anxiety disorders and chronic pain. They modify the metabolism of the body's own neurotransmitters, primarily those of serotonin and norepinephrine, or react with their receptors.

Psychostimulants increase the activity of the central nervous system. Among other things, they eliminate fatigue, reduce the need for sleep, increase the ability to concentrate and inhibit the appetite.
This parameter set also includes psychostimulants such as methylphenidate (Ritalin®). The amphetamine-like substance is mainly used in the treatment of attention deficit/hyperactivity disorder (ADHD). Methylphenidate inhibits the reuptake of dopamine and noradrenaline, thus increasing their concentration in the synaptic cleft and enhancing the activity of the sympathetic nervous system. Atomoxetine, which has a chemical structure similar to fluoxetine, is another drug used to treat ADHD. In Germany, atomoxetine is approved for ADHD and is not subject to the controlled-substances law of Germany (Betäubungsmittelgesetz BtMG). Mianserin, reboxetine, and trazodone are prescribed for depression.

 

Product advantages

With the Parameter Set Antidepressants 2/Psychostimulants/EXTENDED in serum/plasma, 20 different drugs can be measured quickly and efficiently using LC-MS/MS. Due to the careful optimisation of all kit components as well as the chromatographic separation, matrix effects (“ion suppression”) are minimised and the robustness of the method is enhanced. Sample preparation is based on a protein precipitation step. The use of stable isotopically labelled (deuterated) and co-eluting internal standards as well as 3PLUS1® multilevel calibrators ensures high precision and the reproducible and reliable quantification of the analytes. The m

The Parameter Set is a part of the Series A modular system, which enables the analysis of all parameters without switching column or changing the mobile phases, thereby minimising required workload in the laboratory. The Basic Kit A contains all components required for sample preparation and all necessary mobile phases. The MasterColumn® A is the analytical column used for the determination of all Series A analytes. Our portfolio contains further MassTox® Parameter Sets

For the analysis you require the MassTox® TDM Basic Kit A, the specifc MassTox® TDM Parameter Set and the analytical column MassTox® TDM MasterColumn® A.

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Parameter

Atomoxetine, Bupropion, Clomethiazole, Dosulepin, erythro-Dihydrobupropion, Hydroxybupropion, Methylphenidate, Mianserin, Milnacipran, Moclobemide, N-Desmethyldosulepin, Opipramol, Reboxetine, Ritalinic acid, threo-Dihydrobupropion, Tianeptine, Tranylcypromine, Trazodone, Vilazodone, Vortioxetine

Analysis Time

Group 1 = 2.8 min, Group 2 = 6 min

Limit of quantification

1.01 – 115 μg/l

Linearity

up to 150 – 12.500 μg/l

Recovery

81 – 113%

Intraassay

CV = 2.0 – 13.7 %

Interassay

CV = 5.3 – 13.2 %

Pre-analytic Treatment

Stability: depending on the specific analyte. Further information can be obtained from the instruction manual. It is mandatory to ship and keep samples frozen when samples have to be measured for methylphenidate and bupropione.

Sample Preparation

  • Add 800 μl Internal Standard mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 μl sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 μl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 μl of mixture A and mix 30 s minimum (vortex) and centrifuge 5 min.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity

Sample Stability

Prepared samples are stable up to 3 days at ambient temperature and up to 5 days at +2 to +8 °C. For longer storage periods keep them frozen below -18 °C.

Injection Volume

0.2 – 50 µl

Gradient

Group 1 binary, Group 2 isocratic

Ionisation

ESI positive

MS/MS-Mode

Group 1 sMRM, Group 2 MRM

Additional Info

We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Calibrators

Controls