92923 LCMS TDM Series A Antiarrhythmic Drugs

Parameter Set Antiarrhythmic Drugs - LC-MS/MS

Order no.: 92923
  • Acebutolol
  • Ajmaline
  • Amiodarone
  • Aprindine
  • Atenolol
  • Bisoprolol
  • Debutyldronedarone
  • Desethylamiodarone
  • Diltiazem
  • Disopyramide
  • Dronedarone
  • Flecainide
  • Flunarizine
  • Gallopamil
  • Hydroquinidine
  • Lidocaine
  • Metoprolol
  • Mexiletine
  • Norverapamil
  • Propafenone
  • Propranolol
  • Quinidine
  • Sotalol
  • Tocainide
  • Verapamil

Encompasses 25 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the MassTox® TDM Series A

Description

Clinical relevance

Antiarrhythmic drugs are used for the treatment of cardiac arrhythmia to reduce their frequency or intensity. The severity of ventricular and supraventricular rhythm disturbances ranges from harmless extra beats through to complex multiple blows and life-threatening tachycardia. By using antiarrhythmic agents, it is possible to restore a normal electrical cardiac activity. Since antiarrhythmic drugs are an inhomogeneous group, in their chemistry and mechanisms of action, various side effects and drug interactions have to be considered. Their use should be monitored by ECG monitoring, electrolyte and plasma level determinations, in particular when starting new drug regimens. As each antiarrhythmic drug possesses a pro-arrhythmic potential, it also can cause cardiac arrhythmia. Furthermore, there are drug-drug interactions between the different antiarrhythmics, and therefore, combinations should be taken with extreme caution as well as with due attention to the side-effects and interaction profiles. For these reasons cardiac arrhythmia are often only treated with medication when they are very dangerous or associated with a high burden for the patient.

 

Product advantages

With the Parameter Set Antiarrhythmics in serum/plasma, 25 different drugs can be measured quickly and efficiently using LC-MS/MS. By carefully optimising all of the kit components and the chromatographic separation, matrix effects (“ion suppression”) have been minimised and the robustness of the method has been enhanced. Sample preparation is based on protein precipitation. The use of stable isotopically labelled (deuterated), co-eluting internal standards as well as the 3PLUS1® multilevel calibrator guarantees high precision and reliable quantification. The method is comprehensively validated.

The Parameter Set is a part of the Series A modular system, which enables the analysis of all parameters without switching column or changing the mobile phases, thereby minimising required workload in the laboratory. The Basic Kit A contains all components required for sample preparation and all necessary mobile phases. The MasterColumn® A is the analytical column used for the determination of all Series A analytes. Our portfolio contains further MassTox® Parameter Sets

For the analysis you require the MassTox® TDM Basic Kit A, the specifc MassTox® TDM Parameter Set and the analytical column MassTox® TDM MasterColumn® A.

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Parameter

Acebutolol, Ajmaline, Amiodarone, Aprindine, Atenolol, Bisoprolol, Debutyldronedarone, Desethylamiodarone, Diltiazem, Disopyramide, Dronedarone, Flecainide, Flunarizine, Gallopamil, Hydroquinidine, Lidocaine, Metoprolol, Mexiletine, Norverapamil, Propafenone, Propranolol, Quinidine, Sotalol, Tocainide, Verapamil

Analysis Time

1.2 – 3.5 min

Limit of quantification

1.5 – 80 µg/l

Linearity

up to 30000 µg/l

Recovery

87 – 109 %

Intraassay

CV = 2 – 9 %

Interassay

CV = 3 – 8.5 %

Specimen

Serum/Plasma

Sample Preparation

  • Reconstitute the Internal Standard Mix
  • Add 800 µl Internal Standard Mix to 12 ml Precipitation Reagent (= mixture A).
  • Pipette 50 µl sample/calibrator/MassCheck® control into a 1.5 ml reaction vial.
  • Add 25 µl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 µl of mixture A, mix 30 s (vortex) and centrifuge 5 min.
  • Dilute supernatant with Dilution Buffer (depending on instrument sensitivity) and inject into LC-MS/MS system.

Sample Stability

Samples are stable up to 3 days at +2 to +8 °C. For longer storage periods keep samples frozen below -18 °C.

Injection Volume

0.2 – 50 µl

Gradient

Group 1
isocratic 25 % Mobile Phase 2
Group 2
isocratic 43 % Mobile Phase 2
Group 3
isocratic 65 % Mobile Phase 2
Group 4
0.00–0.40 min      0 % Mobile Phase 2
0.41–1.00 min    80 % Mobile Phase 2
1.01–2.90 min  100 % Mobile Phase 2
2.91–3.50 min       0 % Mobile Phase 2

Ionisation

ESI positive

MS/MS-Mode

MRM

Additional Info

We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Calibrators

Calibrators available separately

Controls

Controls available separately