92922 LCMS TDM Series A Antimycotic Drugs-Extended

Parameter Set Antimycotic Drugs/EXTENDED - LC-MS/MS

Order no.: 92922/XT
  • 5-Flucytosine
  • Amphotericin B
  • Anidulafungin
  • Caspofungin
  • Fluconazole
  • Hydroxyitraconazole
  • Isavuconazole
  • Itraconazole
  • Ketoconazole
  • Micafungin
  • Posaconazole
  • Voriconazole

Extended parameter menu of now 12 analytes
Additional internal standards for enhanced robustness
Excellent stability of all componentes
Part of the continuously updated MassTox® TDM Series A

Description

Clinical relevance

Due to the increasing number of immunocompromised patients, invasive fungal infections have been growing steadily in recent years. The immune deficiencies are usually the result of an infectious disease such as AIDS, or are caused by the use of drugs such as immunosuppressants and chemotherapeutics. Most fungal infections are caused by Candida and Aspergillus species, which enter the body either from the air or via injuries to skin. Through improved diagnostic methods rare mycoses such as fusarium, scedosporium and zygomycosis have also become recognised and are accessible for therapy. These infections can be treated with modern antimycotic agents such as posaconazole and voriconazole. But also older agents such as fluconazole or 5-flucytosine, which are both listed in the "WHO Model List of Essential Medicines", are still used in the treatment of fungal infections.

 

Product advantages

With the Parameter Set for the LC-MS/MS analysis of antimycotic drugs/EXTENDED in serum/plasma, 12 different drugs can be measured quickly and efficiently. Due to the careful optimisation of all kit components as well as the chromatographic separation, matrix effects (“ion suppression”) are minimised and the robustness of the method is enhanced. Sample preparation is based on a protein precipitation step. The use of stable isotopically labelled (deuterated) and co-eluting internal standards as well as 3PLUS1® multilevel calibrators ensures high precision and the reproducible and reliable quantification of the analytes. The method is comprehensively validated.

The Parameter Set is a part of the Series A modular system, which enables the analysis of all parameters without switching column or changing the mobile phases, thereby minimising required workload in the laboratory. The Basic Kit A contains all components required for sample preparation and all necessary mobile phases. The MasterColumn® A is the analytical column used for the determination of all Series A analytes. Our portfolio contains further MassTox® Parameter Sets

For the analysis you require the MassTox® TDM Basic Kit A, the specifc MassTox® TDM Parameter Set and the analytical column MassTox® TDM MasterColumn® A.

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Parameter

5-Flucytosine, Amphotericin B, Anidulafungin, Caspofungin, Fluconazole, Hydroxyitraconazole, Isavuconazole, Itraconazole, Ketoconazole, Micafungin, Posaconazole, Voriconazole

Analysis Time

3.2 min

Limit of quantification

0.01 – 1.33 mg/l

Linearity

up to 250 mg/l

Intraassay

CV = 1.3 – 11,2 %

Interassay

CV = 2.3 – 11.6 %

Specimen

Serum/Plasma

Sample Preparation

  • Add 800 µl Internal Standard Mix to 12 ml Precipitation Reagent (= mixture A).
  • Pipette 50 µl sample/calibrator/MassCheck® control into a 1.5 ml reaction vial.
  • Add 25 µl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 µl of mixture A, mix 30 s (vortex) and centrifuge 5 min.
  • Dilute supernatant with Dilution Buffer (depending on instrument sensitivity) and inject into LC-MS/MS system.

Injection Volume

1 – 20 µl

Gradient

binary

Ionisation

ESI positive and negative, polarity switch possible

MS/MS-Mode

MRM

Additional Info

We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Calibrators

Calibrators available separately

Controls