Antibiotics in Serum/Plasma 61000

Antibiotics in Serum/Plasma - HPLC

Order no.: 61000
100 Tests
  • Ampicillin
  • Cefepime
  • Ceftazidime
  • Linezolid
  • Meropenem
  • Piperacillin
  • Sulbactam
  • Tazobactam

Simple to perform tests
One column and one sample prep for all parameters
Stable calibrators and controls available

Description

The assay antibiotics in serum/plasma is an in vitro diagnostic product for the use in clinical laboratories. It is used for the quantitative determination of ampicillin, cefepime, ceftazidime, linezolid, meropenem and piperacillin in human serum and plasma samples for monitoring patients’ blood levels of these antibiotics. The calibrators and controls also include Sulbactam* and Tazobactam* for obtaining qualitative data (customer validation required).

The HPLC determination with subsequent UV detection is done in two groups, the first run via a mobile phase gradient, the second run isocratically. Two internal standards and stable matrix products ensure precise quantification. The additionally included Priming Solution increases sample stability notably.

* Qualitative information on Sulbactam and Tazobactam can be obtained, but data are not CE-IVD compliant.

Chromatogram

Technical Data

Method of Analysis

HPLC

Number of Tests

100

Parameter

Ampicillin, Cefepime, Ceftazidime, Linezolid, Meropenem, Piperacillin, Sulbactam, Tazobactam

Analysis Time

13 min (Group 1), 5 min (Group 2)

Limit of quantification

0.5–2.2 mg/l

Linearity

up to 60–400 mg/l

Recovery

91–104 %

Intraassay

CV = 0.5–1.6 %

Interassay

CV = 2.5–5.5 %

Information

Autosampler temperature: 10 °C

Specimen

Serum/Plasma

Pre-analytic Treatment

Detailed information about the stability of patient samples can be found in the instruction manual.

Sample Preparation

  • Pipette 100 μl sample into a 1.5 ml reaction vial.
  • Add 20 μl Priming Solution, mix briefly (vortex).
  • Add 200 μl Internal Standard, mix 1 min (vortex), centrifuge 5 min.
  • Transfer 100 μl supernatant into autosampler vial with insert.
  • Add 100 μl Dilution Buffer, mix briefly (vortex).
  • Inject 5 μl (group 1) or 10 μl (group 2) into HPLC system.

Sample Stability

The prepared samples are stable at room temperature for 10 hours, at +2 to +8 °C for 2 days and below -18 °C for 4 days, light-protected in closed glass vials.

Additional Info

A binary HPLC gradient system with coolable injector, coolable column oven and UV-detector is required.

Injection Volume

5 μl (Group 1), 10 μl (Group 2)

Flow rate

1 ml/min

Gradient

binary (Group 1)
isocratic (Group 2)

Column temperature

22 °C

Wavelength

0–5.9 min 290 nm, then 210 nm (Group 1)
252 nm (Group 2)

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Mobile Phase A


Order no.: 61001

Mobile Phase B


Order no.: 61002

Internal Standard Mix


Order no.: 61004

Dilution Buffer


Order no.: 61005

Priming Solution


Order no.: 61012

Calibrators

Calibrators available separately

Controls

Controls available separately