22000-HR HPLC kit antiepileptic drugs serum plasma

Antiepileptic Drugs in Serum/Plasma, HIGH RESOLUTION - HPLC

Order no.: 22000/HR
100 Tests
  • 10-OH-carbamazepine
  • Carbamazepine
  • Ethosuximide
  • Lamotrigine
  • Oxcarbamazepine (Trileptal®)
  • Phenobarbital
  • Phenytoin
  • Primidone
  • Sultiame
  • Zonisamide

Analysis of all relevant antiepileptic drugs in a single run
Fast sample preparation: only one precipitation step
Alternatively fast elution (< 5 min) or high resolution chromatographic separation
Metabolite analysis included

Description

Clinical relevance

Epileptic seizures are the result of synchronous discharges of neuron groups in the brain that can lead to sudden and involuntary stereotypical behavioural or sensory disorders. There are numerous types of seizures, each of which requires specialised therapy.

Therapy with anticonvulsive medication (antiepileptics) leads to a reduction in seizures and sometimes even a complete elimination of seizures in most treated patients. The condition is usually patient compliance, e.g. regular use of the medication. Therefore, monitoring of blood levels is essential, especially when starting the use of the medication, as well as for further modification for different life phases. For example, children usually metabolise medication faster than teenagers or adults.

 

Product advantages

  • Analysis of all major antiepileptics in one run
  • Fast sample preparation with only one precipitation step
  • Options for fast elution (< 5 minutes) or high resolution chromatography
  • Measurement of metabolites is possible

 

This Chromsystems AED kit enables the determination of the antiepileptics carbamazepine, ethosuximide, lamotrigin, phenobarbital, phenytoin, primidone, sulthiame, oxcarbazepine (Trileptal®) and zonisamide as well as of the metabolites 10-OH-carbamazepine and carbamazepine-10,11-epoxide in serum/plasma using HPLC. Sample preparation is based on a simple and very efficient precipitation of plasma proteins. Fast chromatography (Fast Elution < 5 minutes) and high resolution chromatography (High Resolution) are available as options for quantifying the analytes.

These analytes as well as other AEDs can also be measured with LC-MS/MS using our Parameter Set for Antiepileptics from the MassTox® Series A.

Chromatogram

Technical Data

Method of Analysis

HPLC

Number of Tests

100

Parameter

10-OH-carbamazepine, Carbamazepine, Ethosuximide, Lamotrigine, Oxcarbamazepine (Trileptal®), Phenobarbital, Phenytoin, Primidone, Sultiame, Zonisamide

Analysis Time

Fast Elution: < 5 min; High Resolution: < 22 min

Linearity

up to 250 mg/l

Recovery

84–112 %

Intraassay

CV < 4 %

Interassay

CV < 6 %

Information

A precolumn has to be used.

Column temperature

ambient (~25 °C)

Flow rate

Fast Elution: 2.0 ml/min; High Resolution: 1.2 ml/min

Specimen

Serum/Plasma

Sample Preparation

  • Pipette 100 µl serum/plasma/calibrator/control and 150 µl Internal Standard into a reaction vial.
  • Mix 2 s (vortex).
  • Add 50 µl Precipitation Reagent.
  • Mix 1 min (vortex).
  • Centrifuge 10-15 min at 9000 x g.
  • Mix 100 µl supernatant with 100 µl Stabilisation Buffer.
  • Inject 20 µl into the HPLC system.

Sample Stability

Stored at +2 to +8 °C for up to 1 week. For longer storage (up to 2 weeks) deep-freeze below -18 °C.

Injection Volume

20 µl

Additional Info

Isocratic HPLC system with UV detector

Wavelength

204 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Calibrators

Controls

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