25000 HPLC kit amiodarone desethylamiodarone serum plasma

Amiodarone and Desethylamiodarone in Serum/Plasma - HPLC

Order no.: 25000
100 Tests
  • Amiodarone
  • Desethylamiodarone


Analysis of active metabolite included
Quick and easy sample preparation
Stable calibration standards and controls

Description

Clinical relevance

Amiodarone is a typical representative of the class III antiarrhythmic agents that induce prolongation of the action potentials and refractory periods in the heart. It is approved in Germany for the treatment of various tachycardia arrhythmias, and is often prescribed in combination with other beta blockers. Additionally, amiodarone is often prescribed when other medications show no effect or stop working. Although amiodarone itself rarely causes cardiac arrhythmias, adverse effects may occur in other organs, e.g. reversible sensitivity to light in the eyes and the skin with risk of sunburn, increased liver-related values, as well as very rarely lung damages and hyperthyroidism/hypothyroidism. Regular monitoring of the amiodarone levels is therefore essential. Its bioactive metabolite desethylamiodarone can also be used for measurements.

 

Product advantages

  • Metabolite analysis included
  • Simple and fast sample preparation
  • Stable internal standards and quality controls

 

This kit allows for the simple and reliable HPLC analysis of amiodarone and desethylamiodarone. Sample preparation is based on a simple and very efficient method of precipitation of all of the interfering components of the sample matrix. The separation is then performed on an isocratic HPLC system with UV detector.

The MassTox® Series A Parameter Set for Antiarrhythmic Drugs is also available for using LC-MS/MS to analyse amiodarone and desethylamiodarone.

Chromatogram

Technical Data

Method of Analysis

HPLC

Number of Tests

100

Parameter

Amiodarone, Desethylamiodarone

Analysis Time

approx. 14 min

Limit of quantification

0.15 mg/l (amiodarone)
0.10 mg/l (desethylamiodarone)

Linearity

up to 20 mg/l

Recovery

99–108 %

Intraassay

CV = 0.5–1.1 %

Interassay

CV = 3.4–5.5 %

Column temperature

ambient (~25 °C)

Flow rate

1 ml/min

Specimen

Serum/Plasma

Pre-analytic Treatment

Patient samples are stable cooled (4 °C) in the dark for at least 24 hours. For storage periods longer than 24 hours patient samples should be frozen at approx. -20 °C.

 

Sample Preparation

  • Pipette 100 µl serum/plasma
    + 150 µl Internal Standard
    + 100 µl Precipitation Reagent into an amber coloured reaction vial (light protection).
  • Mix 20 s (vortex).
  • Centrifuge 10 min at at least 9000 x g.
  • Inject 25 µl of the supernatant into the HPLC system.

Sample Stability

At +2 to +8 °C up to 3 days, freeze for longer storage. Do not use gel separators.

Injection Volume

25 µl

Additional Info

For the HPLC analysis of amiodarone/desethylamiodarone any isocratic HPLC system with UV detector is suitable.

Wavelength

242 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Calibrators

Calibrators available separately

Controls

Controls available separately

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