72000-C steroids serum plasma sample clean up columns

New

MassChrom® Steroids in Serum/Plasma with Sample Clean Up Columns - LC-MS/MS

Order no.: 72072/C
96 Tests
  • 11-Deoxycorticosterone
  • 11-Deoxycortisol
  • 17-OH-progesterone
  • 21-Deoxycortisol
  • Aldosterone
  • Androstenedione
  • Corticosterone
  • Cortisol
  • Cortisone
  • Dehydroepiandrosterone (DHEA)
  • Dehydroepiandrosterone sulfate (DHEA-S)
  • Dihydrotestosterone (DHT)
  • Estradiol
  • Progesterone
  • Testosterone

Optimised and extended method
15 Steroids – 2 panels – 1 analytical column
With Sample Clean Up Columns

Description

Clinical relevance

Steroid hormones are structurally derived from cholesterol and play a role in a wide range of physiological processes, including carbohydrate metabolism, fat and protein metabolism as well as in stress response. They are secreted by the adrenal cortex, testes, ovaries, and during pregnancy by the placenta. They are transported through the bloodstream to the cells of various organs where they influence and regulate the organs’ physiological functions.

The determination of hormones is important for the diagnosis of a number of diseases such as adrenogenital syndrome, hyperaldosteronism, or tumours. By using LC-MS/MS, not only high sensitivity and specificity can be achieved, but also the efficient measurement of multiple analytes in a single run.

 

Product advantages

  • 15 steroids – 2 panels – 1 analytical column
  • One sample preparation for all parameters
  • Clinically most relevant steroids covered
  • Each parameter safeguarded by its own internal standard

 

This optimised and extended MassChrom® kit allows the quantitative determination of 15 steroids in serum/plasma by LC-MS/MS and includes the clinically most important parameters. Sample preparation is the same for both steroid panels using an optimised and efficient solid phase extraction with individual SPE columns (Sample Clean Up Columns). The use of stable isotope-labelled internal standards for every single analyte ensures reproducible and reliable quantitation of the steroids. The method does not require a high-end LC-MS/MS system to be carried out.

Alternatively, there is also a method available using 96 SPE well plates (72072/96, 72072/480).

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Number of Tests

96

Parameter

11-Deoxycorticosterone, 11-Deoxycortisol, 17-OH-progesterone, 21-Deoxycortisol, Aldosterone, Androstenedione, Corticosterone, Cortisol, Cortisone, Dehydroepiandrosterone (DHEA), Dehydroepiandrosterone sulfate (DHEA-S), Dihydrotestosterone (DHT), Estradiol, Progesterone, Testosterone

Analysis Time

Steroid Panel 1: 10.0 min; Steroid Panel 2: 11.7 min

Limit of quantification

Steroid Panel 1: down to 14 ng/l
Steroid Panel 2: down to 5 ng/l

Linearity

Steroid Panel 1: covering reference range of each steroid
Steroid Panel 2: covering reference range of each steroid

Recovery

Steroid Panel 1: 86-115 %; Steroid Panel 2: 76-118 %

Intraassay

Steroid Panel 1: CV 1-6 %
Steroid Panel 2: CV 1-13 %

Interassay

Steroid Panel 1: CV 3-11 %
Steroid Panel 2: CV 4-13 %

Gradient

binary

Column temperature

Steroid Panel 1: 32 °C, Steroid Panel 2: 27 °C

Specimen

Serum/Plasma

Sample Preparation

  • Put Sample Clean Up Columns into tubes for sample preparation.
  • Pipette 0.8 ml Equilibration Reagent 1 into each Sample Clean Up Column, centrifuge and repeat with Equilibration Reagent 2.
  • Pipette 500 μl sample/calibrator/MassCheck® control, 50 μl Internal Standard Mix and 450 μl Extraction Buffer into each Sample Clean Up Column, mix briefly (vortex) and centrifuge.
  • Add 2 x 0.7 ml Wash Buffer and centrifuge.
  • Put autosampler vials into tubes for sample preparation, then put Sample Clean Up Columns on top.
  • Add 1 ml Elution Buffer and centrifuge.
  • Evaporate eluates in the autosampler vials (nitrogen or compressed air) to dryness.
  • Add 100 μl Reconstitution Buffer.
  • Close autosampler vials, mix briefly (vortex) and inject 5–50 μl of the eluate. 

Sample Stability

The stability depends on the specific steroid. Further information can be obtained from the instruction manual.

Injection Volume

5-50 µl

Ionisation

ESI negative and positive (ESI switch)

MS/MS-Mode

MRM

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Calibrators

Controls

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