43000 HPLC kit hippuric acid methylhippuric acid mandelic acid phenylglyoxylic acid urine

Hippuric Acid, Methylhippuric Acids, Mandelic Acid and Phenylglyoxylic Acid in Urine - HPLC

Order no.: 43000
100 Tests
  • Hippuric acid
  • m-Methylhippuric acid
  • Mandelic acid
  • o-Methylhippuric acid
  • p-Methylhippuric acid
  • Phenylglyoxylic acid

Only 10 µl sample volume
Internal standard included
Simple sample preparation


Clinical relevance

Hippuric acid, o-, m-, p-methylhippuric acid, mandelic acid and phenylglyoxylic acid are the main metabolites of the organic solvents: toluene, xylene, and styrene. These toxic compounds are widely used throughout various industries, making the determination of their concentrations relevant to occupational health in a number of different professional fields. Due to their high levels of volatility, they primarily enter the body through inhalation and are rapidly absorbed through the lungs. For occupational health monitoring of people who are exposed to these compounds at work, measurement of the metabolites in urine is more reliable than determining the concentrations of the toxins in the blood, due to their very fast metabolism rates.

For any hazardous substance or its metabolite, a BTV (Biological Tolerance Value) is defined as a threshold value for maximum absorption in biological material. Solvent-related harm to human health can be prevented by performing regular monitoring of urine.


Product advantages

  • Only 10 µl sample volume
  • Optimised internal standard
  • Straightforward sample preparation


This Chromsystems kit provides rapid and reliable determination of the main metabolites of toluene, xylene, and styrene in a single run. A 10 µl urine sample is all that is required, which is then analysed using an isocratic HPLC system with UV detection following a simple and fast preparation step. The inclusion of a tailor-made internal standard reduces analytical variations and guarantees high precision and reliability of results.


Technical Data

Method of Analysis


Number of Tests



Hippuric acid, m-Methylhippuric acid, Mandelic acid, o-Methylhippuric acid, p-Methylhippuric acid, Phenylglyoxylic acid

Analysis Time

approx. 20 min

Limit of quantification

15 mg/l


Hippuric acid: up to 18000 mg/l Methylhippuric acid: up to 7000 mg/l Mandelic acid: up to 4000 mg/l Phenylglyoxylic acid: up to 1700 mg/l


94-107 %


CV < 3 %


CV < 7 %

Column temperature

ambient (~25 °C)

Flow rate

1.0 ml/min

BEI values

Hippuric acid: 1500 mg/l; Methylhippuric acid: 2000 mg/l; Mandelic acid: 400 mg/l; Phenylglyoxylic acid: 100 mg/l



Pre-analytic Treatment

Time of sample drawing

  • After end of shift


Sample Preparation

  • Pipette 1000 µl Internal Standard into a reaction vial.
  • Add 10 µl urine, vortex briefly.
  • Centrifuge 5 min at 9000 g.
  • Inject 20 µl supernatant into the HPLC system.

Sample Stability

At +2 to +8 °C up to 1 week, at -20 °C up to 3 months. Avoid repeated freeze/thaw cycles.

Injection Volume

20 µl

Additional Info

For the Chromsystems HPLC analysis of occupational medicine parameters in urine any isocratic HPLC system with UV detector is suitable.


207 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately


Calibrators available separately


Controls available separately