67000 HPLC kit malondialdehyde plasma serum

Malondialdehyde in Serum/Plasma - HPLC

Order no.: 67000
100 Tests
  • Malondialdehyde

The marker of lipid peroxidation
Trouble-free analysis
Reliable results

Description

Clinical relevance

Excessive oxidative stress can lead to degenerative damages in the body, such as oxidation of lipids, proteins, and DNA. The body has multiple endogenous biomarkers that can provide information about its physical condition, such as β-carotene, coenzyme Q10, glutathione, vitamin C and malondialdehyde (MDA), of which an increase in serum or plasma is a sign of oxidative stress.

MDA is an oxidation product of polyunsaturated fatty acids, and thus the laboratory diagnostic marker used for lipid peroxidation. Recent clinical studies show that the MDA concentration is elevated in patients with cystic fibrosis and other chronic respiratory diseases. The additional determination of the MDA level together with other parameters provides useful information about the degree of oxidative stress to optimise the therapy.

 

Product advantages

  • The marker for lipid peroxidation
  • Interference-free analysis
  • Reliable results

 

This Chromsystems kit allows the reliable chromatographic determination of malondialdehyde in one isocratic HPLC run with fluorescence detection. Sample preparation is based on an effective protein precipitation step followed by derivatisation. The resulting fluorophore is specific and detectable even at very low concentrations.

Chromatogram

Technical Data

Method of Analysis

HPLC

Number of Tests

100

Parameter

Malondialdehyde

Analysis Time

< 5 min

Limit of quantification

0.01 µmol/l

Linearity

up to 10 µmol/l

Recovery

99 %

Intraassay

CV < 3 %

Interassay

CV < 9 %

Gradient

Isocratic

Information

For the Chromsystems HPLC analysis of malondialdehyde any isocratic HPLC system with fluorescence detecor is suitable.

Column temperature

ambient (~25 °C)

Flow rate

1.0 ml/min

Specimen

Plasma/Serum

Pre-analytic Treatment

Specimens should be frozen for transport.

Sample Preparation

  • Mix 100 µl plasma and 500 µl Precipitation Reagent, vortex 10 s.

  • Centifuge 5 min at 16 000 x g.

  • Transfer 500 μl of the supernatant into a labelled derivatisation vial. Add 100 μl Derivatisation Reagent and mix briefly. Incubate 60 min at 95 °C, and cool down

    immediately.

  • Add 500 μl Neutralisation Buffer mix briefly.

  • Inject 20 µl into the HPLC system.

Sample Stability

Storage life is up to 12 h at +2 to +8 °C. For longer storage (up to 4 weeks), deep freeze samples below -18 °C.

Injection Volume

20 µl

Flow

1.0 ml/min

Additional Info

For the Chromsystems HPLC analysis of malondialdehyde any isocratic HPLC system with fluorescence detector is suitable.

Wavelength

EX 515 nm, EM 553 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Calibrators

Calibrators available separately

Controls

Controls available separately

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